FK506 Binding Protein 8 (FKBP8) Recombinant Protein | FKBP8 recombinant protein

Recombinant FK506 Binding Protein 8 (FKBP8)

Cat. Num. AAA2018606

• Gene Names: Fkbp8; 38kDa; FKBP-8; Fkbp38; FKBP-38
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: FK506 Binding Protein 8 (FKBP8); Recombinant FK506 Binding Protein 8 (FKBP8); FKBP8 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF- G QVVTVHLQMS LENGTRVQEE PELAFTLGDC DVIQALDLSV PLMDVGETAM VTADSKYCYG PQGSRSPYIP PHAALCLEVT LKTAEDGPDL EMLSGQERVA LANRKRECGN AHYQRADFVL AANSYDLAIK AITSNTKVDM TCEEEEELLQ LKVKCLNNLA ASQLKLDHYR AALRSCSQVL EHQPDNIKAL FRKGKVLAQQ GEYSEAIPIL RAALKLEPS
• Sequence Length: 403

• Applicable Applications for FKBP8 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Gly110~Ser329 (Accession # O35465) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 161484658
• NCBI GeneID: 14232
• NCBI Accession #: NP_001104536.1
• NCBI GenBank Nucleotide #: NM_001111066.1
• UniProt Accession #: O35465; Q3UK86; Q6GTX9; Q811M7; Q811R4; Q8C2F0; Q99L93
• Molecular Weight: 25.7kDa
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase FKBP8 isoform a
• NCBI Official Synonym Full Names: FK506 binding protein 8
• NCBI Official Symbol: Fkbp8
• NCBI Official Synonym Symbols: 38kDa; FKBP-8; Fkbp38; FKBP-38
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase FKBP8; FKBPR38; mFKBP38; rotamase; 38 kDa FKBP; PPIase FKBP8; FK506-binding protein 8; FK506-binding protein 38; 38 kDa FK506-binding protein
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase FKBP8
• Protein Family: Peptidyl-prolyl cis-trans isomerase
• UniProt Gene Name: Fkbp8
• UniProt Synonym Gene Names: Fkbp38; Sam11; PPIase FKBP8; 38 kDa FKBP; FKBP-38; mFKBP38; FKBP-8
• UniProt Entry Name: FKBP8_MOUSE

Uniprot Description

FKBP8: Constitutively inactive PPiase, which becomes active when bound to calmodulin and calcium. Seems to act as a chaperone for BCL2, targets it to the mitochondria and modulates its phosphorylation state. The BCL2/FKBP8/calmodulin/calcium complex probably interferes with the binding of BCL2 to its targets. The active form of FKBP8 may therefore play a role in the regulation of apoptosis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Isomerase; Chaperone; Membrane protein, integral; EC 5.2.1.8

Cellular Component: mitochondrial envelope; mitochondrion; membrane; integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: identical protein binding; peptidyl-prolyl cis-trans isomerase activity; FK506 binding; isomerase activity

Biological Process: neural tube development; smoothened signaling pathway; protein peptidyl-prolyl isomerization; camera-type eye development; protein folding; apoptosis; multicellular organism growth; regulation of BMP signaling pathway; cell fate specification; dorsal/ventral pattern formation; regulation of gene expression; positive regulation of BMP signaling pathway; dorsoventral neural tube patterning; negative regulation of apoptosis