Rabbit Grp75 Polyclonal Antibody | anti-HSPA9 antibody

Anti-Grp75 antibody

Cat. Num. AAA175719

• Gene Names: HSPA9; CSA; MOT; MOT2; CRP40; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75; HEL-S-124m
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Grp75; Polyclonal Antibody; Anti-Grp75 antibody; Stress-70 protein; mitochondrial; heat shock 70kDa protein 9(mortalin); 75 kDa glucose regulated protein antibody; 75 kDa glucose-regulated protein antibody; CSA antibody; Glucose Regulated Protein antibody; Grp 75 antibody; GRP-75 antibody; GRP75 antibody; GRP75_HUMAN antibody; Heat shock 70 kDa protein 9 antibody; Heat shock 70kD protein 9 antibody; heat shock 70kDa protein 9 antibody; Heat shock 70kDa protein 9B antibody; Heat shock protein 74 kDa A antibody; Heat shock protein A antibody; Heat shock protein cognate 74 antibody; Hsc74 antibody; Hsp74 antibody; Hsp74a antibody; HSPA9 antibody; Hspa9a antibody; HSPA9B antibody; MGC4500 antibody; mitochondrial antibody; Mortalin 2 antibody; Mortalin antibody; Mortalin perinuclear antibody; Mortalin2 antibody; MOT 2 antibody; MOT antibody; MOT2 antibody; Mthsp70 antibody; p66 mortalin antibody; P66 MOT antibody; PBP74 antibody; Peptide binding protein 74 antibody; Peptide-binding protein 74 antibody; Stress 70 protein mitochondrial antibody; Stress 70 protein mitochondrial precursor antibody; Stress-70 protein antibody; anti-HSPA9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 679

• Applicable Applications for anti-HSPA9 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Grp75 (591-609aa DTETKMEEFKDQLPADECN), identical to the related rat and mouse sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-Grp75 antibody, MBS175719, Western blottingAll lanes: Anti Grp75 (MBS175719) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: A549 Whole Cell Lysate at 40ugLane 3: 293T Whole Cell Lysate at 40ugLane 4: M431 Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 74KDObserved bind size: 74KD)

Immunohistochemistry (IHC)

(Anti-Grp75 antibody, MBS175719, IHC(P)IHC(P): Human Lung Cancer Tissue )

Immunohistochemistry (IHC)

(Anti-Grp75 antibody, MBS175719, IHC(P)IHC(P): Rat Liver Tissue )

Immunocytochemistry (ICC)

(Anti-Grp75 antibody, MBS175719, ICCICC: A549 Cell )

Related Product Information for anti-HSPA9 antibody

Description: Rabbit IgG polyclonal antibody for Stress-70 protein, mitochondrial(HSPA9) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.
Background: HSPA9(heat shock 70kDa protein 9(mortalin)), also known as GRP75, mot-2, mthsp75, PBP74,HSPA9B, MORTALIN or MORTALIN, PERINUCLEAR, is a highly conserved member of the HSP70 family of proteins. It functions as a chaperone in the mitochondria, cytoplasm, and centrosome. The HSPA9 gene is mapped to chromosome 5q31.2 based on an alignment of the HSPA9 sequence with the genomic sequence. Knockdown of HSPA9 in erythroid cultures was associated with an increased number of cells in the G0/G1 phase of the cell cycle and accelerated apoptosis. Knockdown of Hspa9 in mouse bone marrow cells, followed by transplantation into wildtype recipients, also resulted in loss of erythroid cell number. Haploinsufficiency for HSPA9 may contribute to abnormal hematopoiesis in myelodysplastic syndromes with deletions spanning chromosome 5q31.2. This protein plays a role in the control of cell proliferation. It may also act as a chaperone.

NCBI and Uniprot Product Information

• NCBI GI #: 21264428
• NCBI GeneID: 3313
• UniProt Accession #: P38646; P30036; P31932; Q1HB43; Q53H23; Q6GU03; Q9BWB7; Q9UC56; B2RCM1
• Molecular Weight: 73,680 Da
• NCBI Official Full Name: Stress-70 protein, mitochondrial
• NCBI Official Synonym Full Names: heat shock 70kDa protein 9 (mortalin)
• NCBI Official Symbol: HSPA9
• NCBI Official Synonym Symbols: CSA; MOT; MOT2; CRP40; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75; HEL-S-124m
• NCBI Protein Information: stress-70 protein, mitochondrial; mortalin-2; p66-mortalin; mortalin, perinuclear; heat shock 70kD protein 9B; peptide-binding protein 74; 75 kDa glucose-regulated protein; catecholamine-regulated protein 40; epididymis secretory sperm binding protein Li 124m
• UniProt Protein Name: Stress-70 protein, mitochondrial
• Protein Family: Stress-70 protein
• UniProt Gene Name: HSPA9
• UniProt Synonym Gene Names: GRP75; HSPA9B; mt-HSP70; GRP-75; MOT; PBP74
• UniProt Entry Name: GRP75_HUMAN

NCBI Description

This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.[provided by RefSeq, May 2010]

Uniprot Description

HSPA9B: Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone. Interacts with FXN. Interacts with HSCB. Component of the MINOS/MitOS complex, that includes IMMT, HSPA9 and CHCHD3 and associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2. Belongs to the heat shock protein 70 family.

Protein type: Chaperone; Nucleolus; Heat shock protein

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: focal adhesion; mitochondrion; cytoplasm; nucleolus

Molecular Function: protein binding; ubiquitin protein ligase binding; unfolded protein binding; ATP binding

Biological Process: cellular protein metabolic process; protein folding; protein export from nucleus; protein targeting to mitochondrion; negative regulation of apoptosis