Fish Glucagon (GC) ELISA Kit | GC elisa kit
Fish Glucagon (GC) ELISA Kit
Reactivity
Fish
Synonyms
Glucagon (GC); Fish Glucagon (GC) ELISA Kit; GCG; GLP1; GLP2; GRPP; glicentin-related polypeptide; glucagon-like peptide 1; glucagon-like peptide 2; GC elisa kit
Reactivity
Fish
Specificity
This assay has high sensitivity and excellent specificity for detection of Fish GCG. No significant cross-reactivity or interference between Fish GCG and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Serum, Plasma, Other biological fluids
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C. Please refer to Instruction Manual.
Related Product Information for GC elisa kit
This assay employs a two-site sandwich ELISA to quantitate GCG in samples. An antibody specific for GCG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GCG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GCG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GCG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Glucagon has a major role in maintaining normal concentrations of glucose in blood, and is often described as having the opposite effect of insulin. That is, glucagon has the effect of increasing blood glucose levels. Glucagon is a linear peptide of 29 amino acids. Its primary sequence is almost perfectly conserved among vertebrates, and it is structurally related to the secretin family of peptide hormones. Glucagon is synthesized as proglucagon and proteolytically processed to yield glucagon within alpha cells of the pancreatic islets. Proglucagon is also expressed within the intestinal tract, where it is processed not into glucagon, but to a family of glucagon-like peptides (enteroglucagon).
Overview: Glucagon has a major role in maintaining normal concentrations of glucose in blood, and is often described as having the opposite effect of insulin. That is, glucagon has the effect of increasing blood glucose levels. Glucagon is a linear peptide of 29 amino acids. Its primary sequence is almost perfectly conserved among vertebrates, and it is structurally related to the secretin family of peptide hormones. Glucagon is synthesized as proglucagon and proteolytically processed to yield glucagon within alpha cells of the pancreatic islets. Proglucagon is also expressed within the intestinal tract, where it is processed not into glucagon, but to a family of glucagon-like peptides (enteroglucagon).
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Product Notes
The Fish GC (Catalog #AAA2801548) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2801548 ELISA Kit recognizes Fish GC. It is sometimes possible for the material contained within the vial of "Glucagon (GC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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