Chicken Antithrombin-III ELISA Kit | SERPINC1 elisa kit

Chicken Antithrombin-III ELISA Kit

Cat. Num. AAA288426

• Reactivity: Chicken
• Synonyms: Antithrombin-III; Chicken Antithrombin-III ELISA Kit; SERPINC1 elisa kit

• Reactivity: Chicken
• Specificity: This assay recognizes recombinant and natural chicken ATIII . No significant cross-reactivity or interference was observed.
• Sequence Length: 464

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Detection Range: 7.80 ng/ml -500 ng/ml.
• Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of chicken ATIII concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SERPINC1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATIII . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ATIII and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ATIII , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ATIII in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 461514
• UniProt Accession #: Q03352
• Molecular Weight: 11,281 Da
• NCBI Official Full Name: Antithrombin-III
• UniProt Protein Name: Antithrombin-III
• Protein Family: Antithrombin
• UniProt Gene Name: SERPINC1
• UniProt Synonym Gene Names: AT3; ATIII
• UniProt Entry Name: ANT3_CHICK

Uniprot Description

Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin ().