Canine Fibrinogen ELISA Kit | Fb elisa kit

Canine Fibrinogen ELISA Kit

Cat. Num. AAA739809

• Reactivity: Canine
• Synonyms: Fibrinogen; Canine Fibrinogen ELISA Kit; Fb elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 10-250ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This Fb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine Fb. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Fb elisa kit

Principle of the Assay: Fb ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Fb. Standards or samples are then added to the microtiter plate wells and Fb if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Fb present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for Fb are added to each well to "sandwich" the Fb immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Fb and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Fb concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Fb elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 350
• NCBI GeneID: 280792
• UniProt Accession #: P12799
• Molecular Weight: 50,244 Da
• NCBI Official Full Name: fibrinogen
• NCBI Official Synonym Full Names: fibrinogen gamma chain<
• NCBI Official Symbol: FGG
• NCBI Protein Information: fibrinogen gamma-B chain; gamma'; fibrinogen, gamma polypeptide
• UniProt Protein Name: Fibrinogen gamma-B chain
• Protein Family: Fibrinogen
• UniProt Gene Name: FGG
• UniProt Synonym Gene Names: Gamma'
• UniProt Entry Name: FIBG_BOVIN

Uniprot Description

Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.