Fibrinogen Alpha (FGa) Recombinant Protein | FGa recombinant protein

Recombinant Fibrinogen Alpha (FGa)

Cat. Num. AAA2011025

• Gene Names: Fga; Fib; ENSMUSG00000059807
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Fibrinogen Alpha (FGa); Recombinant Fibrinogen Alpha (FGa); FGa recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF-WGVFSEF GDSSSPATRK EYHTGKAVTS KGDKELLIGK EKVTSSGTST THRSCSKTIT KTVTGPDGRR EVVKEVITSD DGSDCGDATE LDISHSFSGS LDELSERHPD LSGFFDNHFG LISPNFKEFG SKTHSDSDIL TNIEDPSSHV PEFSSSSKTS TVKKQVTKTY KMADEAGSEA HREGETRNTK RGRARARPTR DCD
• Sequence Length: 789

• Applicable Applications for FGa recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Trp357~Asp556 (Accession # E9PV24) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 167555029
• NCBI GeneID: 14161
• NCBI Accession #: NP_001104518.1
• NCBI GenBank Nucleotide #: NM_001111048.1
• UniProt Accession #: E9PV24
• Molecular Weight: 23.3kDa
• NCBI Official Full Name: fibrinogen, alpha polypeptide isoform 1
• NCBI Official Synonym Full Names: fibrinogen alpha chain
• NCBI Official Symbol: Fga
• NCBI Official Synonym Symbols: Fib; ENSMUSG00000059807
• NCBI Protein Information: fibrinogen, alpha polypeptide; fibrinogen, A alpha polypeptide
• UniProt Protein Name: Protein Fga
• Protein Family: Fibrinogen
• UniProt Gene Name: Fga
• UniProt Entry Name: E9PV24_MOUSE

NCBI Description

This gene encodes a subunit of the coagulation factor fibrinogen, which is a component of the blood clot. The encoded protein is proteolytically processed by thrombin during the conversion of fibrinogen to fibrin. Mice lacking the encoded protein display bleeding in the peritoneal cavity, skin and soft tissues around joints immediately after birth, and are predisposed to spontaneous fatal abdominal hemorrhage as they grow. Pregnant mice lacking the encoded protein succumb to uterine bleeding during gestation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Uniprot Description

FGA: Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. Defects in FGA are a cause of congenital afibrinogenemia (CAFBN). This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha- dysfibrinogenemias. Defects in FGA are a cause of amyloidosis type 8 (AMYL8); also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cell surface; rough endoplasmic reticulum; fibrinogen complex; cytoplasm; extracellular region; cell cortex; vesicle; external side of plasma membrane

Molecular Function: protein binding, bridging; cell adhesion molecule binding; structural molecule activity; receptor binding

Biological Process: protein polymerization; platelet activation; cellular protein complex assembly; positive regulation of heterotypic cell-cell adhesion; positive regulation of protein secretion; immune system process; hemostasis; cell-matrix adhesion; positive regulation of vasoconstriction; innate immune response; signal transduction; response to calcium ion; blood coagulation; positive regulation of exocytosis