Coagulation Factor V (F5) Recombinant Protein | F5 recombinant protein

Recombinant Coagulation Factor V (F5)

Cat. Num. AAA2010086

• Gene Names: F5; Cf5; Cf-5; AI173222
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Coagulation Factor V (F5); Recombinant Coagulation Factor V (F5); F5 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF- GLRMY EQEWVRLHLL NMGGSRDIHV VHFHGQTLLD NRTKQHQLGV WPLLPGSFKT LEMKASKPGW WLLDTEVGEN QVAGMQTPFL IIDKECKMPM GLSTGVISDS QIKASEYLTY WEPRLARLNN AGSYNAWSIE KTALDFPIKP WIQVDMQKEV VVTGIQTQGA KHYLKSCFTT EFQVAYSSDQ TNWQIFRGKS GKSVMYFTGN SDGSTIKENR LDPPIVARYI RIHPTKSYNR PTLRLELQGC EVNGCSTP
• Sequence Length: 2183

• Applicable Applications for F5 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Gly1776~Pro2028 (Accession # O88783) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6679731
• NCBI GeneID: 14067
• NCBI Accession #: NP_032002.1
• NCBI GenBank Nucleotide #: NM_007976.2
• UniProt Accession #: O88783; Q3UTQ2; Q3UV80
• Molecular Weight: 30.3kDa
• NCBI Official Full Name: coagulation factor V
• NCBI Official Synonym Full Names: coagulation factor V
• NCBI Official Symbol: F5
• NCBI Official Synonym Symbols: Cf5; Cf-5; AI173222
• NCBI Protein Information: coagulation factor V; activated protein C cofactor
• UniProt Protein Name: Coagulation factor V
• Protein Family: Coagulation factor
• UniProt Gene Name: F5
• UniProt Entry Name: FA5_MOUSE

NCBI Description

This gene encodes a glycoprotein coagulation factor that plays a critical role in the process of blood coagulation and hemostasis. The encoded protein is activated by thrombin, to generate a heterodimer containing heavy and light chains held together by calcium ions. About half of the mice lacking the encoded protein die at an embryonic stage possible due to abnormal yolk-sac vasculature while the remaining animals succumbed to massive hemorrhage immediately after birth. A point mutation in this gene has been shown to cause disseminated intravascular thrombosis in the perinatal period, resulting in frequent deaths of newborn mice. [provided by RefSeq, Apr 2015]

Uniprot Description

factor V: Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin. Defects in F5 are the cause of factor V deficiency (FA5D); also known as Owren parahemophilia. It is an hemorrhagic diastesis. Defects in F5 are the cause of thrombophilia due to activated protein C resistance (THPH2). THPH2 is a hemostatic disorder due to defective degradation of factor Va by activated protein C. It is characterized by a poor anticoagulant response to activated protein C resulting in tendency to thrombosis. Defects in F5 are a cause of susceptibility to Budd- Chiari syndrome (BDCHS). A syndrome caused by obstruction of hepatic venous outflow involving either the hepatic veins or the terminal segment of the inferior vena cava. Obstructions are generally caused by thrombosis and lead to hepatic congestion and ischemic necrosis. Clinical manifestations observed in the majority of patients include hepatomegaly, right upper quadrant pain and abdominal ascites. Budd-Chiari syndrome is associated with a combination of disease states including primary myeloproliferative syndromes and thrombophilia due to factor V Leiden, protein C deficiency and antithrombin III deficiency. Budd-Chiari syndrome is a rare but typical complication in patients with polycythemia vera. Defects in F5 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in F5 are associated with susceptibility to pregnancy loss, recurrent, type 1 (RPRGL1). RPRGL1 is a common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Belongs to the multicopper oxidase family.

Protein type: Protease; Secreted; Secreted, signal peptide

Cellular Component: Golgi apparatus; extracellular space; membrane; endoplasmic reticulum; extracellular region; vesicle

Molecular Function: copper ion binding; serine-type endopeptidase activity; metal ion binding

Biological Process: hemostasis; blood circulation; proteolysis; blood coagulation