Iduronate-2-Sulfatase (IDS) Recombinant Protein | IDS recombinant protein

Recombinant Iduronate-2-Sulfatase (IDS)

Cat. Num. AAA2011812

• Gene Names: Ids; AW214631
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Iduronate-2-Sulfatase (IDS); Recombinant Iduronate-2-Sulfatase (IDS); IDS recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF-L HANLLCPVDV ADVPEGTLPD KQSTEEAIRL LEKMKTSASP FFLAVGYHKP HIPFRYPKEF QKLYPLENIT LAPDPHVPDS LPPVAYNPWM DIREREDVQA LNISVPYGPI PEDFQRKIRQ SYFASVSYLD TQVGHVLSAL DDLRLAHNTI IAFTSDHGWA LGEHGEWAKY SNFDVATRVP LMLYVPGRTA PLPAAGQKLF PYRDPFDPAS DWMDAGRHTE DLVELVSLFP TLAGLAGLPV PPRCPIPSFH VELCREGQNL QKHLQLHD
• Sequence Length: 552

• Applicable Applications for IDS recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Leu180~Asp448 (Accession # Q08890) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 84781812
• NCBI GeneID: 15931
• NCBI Accession #: NP_034628.2
• NCBI GenBank Nucleotide #: NM_010498.3
• UniProt Accession #: Q08890; Q32KI7; Q3TM30
• Molecular Weight: 31.8kDa
• NCBI Official Full Name: iduronate 2-sulfatase
• NCBI Official Synonym Full Names: iduronate 2-sulfatase
• NCBI Official Symbol: Ids
• NCBI Official Synonym Symbols: AW214631
• NCBI Protein Information: iduronate 2-sulfatase; iduronate sulfatase; iduronate-2-sulfatase; alpha-L-iduronate sulfate sulfatase
• UniProt Protein Name: Iduronate 2-sulfatase
• Protein Family: Iduronate 2-sulfatase
• UniProt Gene Name: Ids
• UniProt Entry Name: IDS_MOUSE

Uniprot Description

IDS: Required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Defects in IDS are the cause of mucopolysaccharidosis type 2 (MPS2); also known as Hunter syndrome. MPS2 is an X-linked lysosomal storage disease characterized by intracellular accumulation of heparan sulfate and dermatan sulfate and their excretion in urine. Most children with MPS2 have a severe form with early somatic abnormalities including skeletal deformities, hepatosplenomegaly, and progressive cardiopulmonary deterioration. A prominent feature is neurological damage that presents as developmental delay and hyperactivity but progresses to mental retardation and dementia. They die before 15 years of age, usually as a result of obstructive airway disease or cardiac failure. In contrast, those with a mild form of MPS2 may survive into adulthood, with attenuated somatic complications and often without mental retardation. Belongs to the sulfatase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.1.6.13; Glycan Metabolism - glycosaminoglycan degradation

Cellular Component: lysosome

Molecular Function: protein binding; hydrolase activity; metal ion binding; iduronate-2-sulfatase activity; sulfuric ester hydrolase activity; catalytic activity

Biological Process: metabolic process