Typical Testing Data/Standard Curve (for reference only)
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Human Fatty acid synthase ELISA Kit | FASN elisa kit
Human Fatty acid synthase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
()
Typical Testing Data/Standard Curve (for reference only)
()
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]
Uniprot Description
FASN: a multifunctional enzyme. Catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
Protein type: EC 2.3.1.39; EC 1.3.1.39; Hydrolase; Acetyltransferase; Lipid Metabolism - fatty acid biosynthesis; EC 1.1.1.100; EC 4.2.1.59; Oxidoreductase; EC 2.3.1.41; EC 2.3.1.38; EC 3.1.2.14; EC 2.3.1.85; Lyase
Chromosomal Location of Human Ortholog: 17q25
Cellular Component: cytoplasm; cytosol; glycogen granule; Golgi apparatus; melanosome; membrane; mitochondrion; plasma membrane
Molecular Function: 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity; 3-oxoacyl-[acyl-carrier-protein] reductase activity; 3-oxoacyl-[acyl-carrier-protein] synthase activity; [acyl-carrier-protein] S-acetyltransferase activity; [acyl-carrier-protein] S-malonyltransferase activity; drug binding; enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity; enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity; fatty-acid synthase activity; myristoyl-[acyl-carrier-protein] hydrolase activity; oleoyl-[acyl-carrier-protein] hydrolase activity; palmitoyl-[acyl-carrier-protein] hydrolase activity; protein binding; protein homodimerization activity
Biological Process: acetyl-CoA metabolic process; cellular lipid metabolic process; energy reserve metabolic process; fatty acid biosynthetic process; fatty acid metabolic process; mammary gland development; osteoblast differentiation; pantothenate metabolic process; positive regulation of cellular metabolic process; triacylglycerol biosynthetic process; vitamin metabolic process; water-soluble vitamin metabolic process
Research Articles on FASN
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Product Notes
The Human FASN fasn (Catalog #AAA2883650) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2883650 ELISA Kit recognizes Human FASN. It is sometimes possible for the material contained within the vial of "Fatty acid synthase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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