Fatty Acid Synthase (FASN) Recombinant Protein | FASN recombinant protein

Recombinant Fatty Acid Synthase (FASN)

Cat. Num. AAA2012077

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Fatty Acid Synthase (FASN); Recombinant Fatty Acid Synthase (FASN); FASN recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF- SKLHMPCY GLQCTKAAPL DSIQSLASYY IDCMKQIQPE GPYRIAGYSF GACVAFEMCS QLQAQQNASH ALNSLFLFDG SHSFVAAYTQ SYRAKLTQGN EAALETEALC AFVQQFTGIE YNKLLEILLP LEDLEARVNA AADLITQIHK NINREALSFA AASFYHKLKA ADKYIPESKY HGNVTLMRAK THNEYEEGLG GDYRLSEVCD GKVSVHIIEG DHRTLLEGDG VESIIGIIHG SLAEPRVSVR EG
• Sequence Length: 2512

• Applicable Applications for FASN recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Chicken (Gallus)
• Expression System: Prokaryotic expression
• Residues: Ser2263~Gly2512 (Accession # P12276) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 1345958
• NCBI GeneID: 396061
• UniProt Accession #: P12276
• Molecular Weight: 29.1kDa
• NCBI Official Full Name: Fatty acid synthase
• NCBI Official Synonym Full Names: fatty acid synthase
• NCBI Official Symbol: FASN
• NCBI Protein Information: fatty acid synthase; thioesterase
• UniProt Protein Name: Fatty acid synthase
• Protein Family: Fatty acid synthase
• UniProt Gene Name: FASN
• UniProt Synonym Gene Names: FAS
• UniProt Entry Name: FAS_CHICK

Uniprot Description

Function: Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.

Catalytic activity: Acetyl-CoA + n malonyl-CoA + 2n NADPH = a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP+.Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein].Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein].Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H2O.An acyl-[acyl-carrier protein] + NADP+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH.Oleoyl-[acyl-carrier-protein] + H2O = [acyl-carrier-protein] + oleate.

Subunit structure: Homodimer which is arranged in a head to tail fashion.

Sequence similarities: Contains 1 acyl carrier domain.

Sequence caution: The sequence AAA82106.1 differs from that shown. Reason: Frameshift at position 2352.