SDS-Page
Semaphorin 3E (SEMA3E) Recombinant Protein | SEMA3E recombinant protein
Recombinant Semaphorin 3E (SEMA3E)
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Semaphorin 3E (SEMA3E); Recombinant Semaphorin 3E (SEMA3E); SEMA3E recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF- LDAGTYFCQT VEHSIVHTVR KITLEIVEEE RVDEMFSKDY EEEISHKMPC PMQSNIPQVS KPWYKEFLQL IGYSNFQRVE EYCEKVWCTD
MGHHHHHHSG SEF- LDAGTYFCQT VEHSIVHTVR KITLEIVEEE RVDEMFSKDY EEEISHKMPC PMQSNIPQVS KPWYKEFLQL IGYSNFQRVE EYCEKVWCTD
Sequence Length
785
Applicable Applications for SEMA3E recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Chicken (Gallus)
Expression System
Prokaryotic expression
Residues
Leu651~Asp740 (Accession # O42237) with N-terminal His-Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12.3kDa
NCBI Official Full Name
semaphorin-3E
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
NCBI Official Symbol
SEMA3E
NCBI Protein Information
semaphorin-3E; COLL-5; collapsin-5
UniProt Protein Name
Semaphorin-3E
Protein Family
UniProt Gene Name
SEMA3E
UniProt Synonym Gene Names
COLL5; COLL-5
UniProt Entry Name
SEM3E_CHICK
Uniprot Description
Function: Plays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections. Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis. Can down-regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis. Induces the collapse and paralysis of neuronal growth cones. Plays an important role in ensuring the specificity of synapse formation
By similarity.
Subcellular location: Secreted.
Tissue specificity: Collapsin-1, -2, -3, and -5 bind to overlapping but distinct axon tracts.
Domain: Strong binding to neuropilin is mediated by the carboxy third of the protein.
Sequence similarities: Belongs to the semaphorin family.Contains 1 Ig-like C2-type (immunoglobulin-like) domain.Contains 1 Sema domain.
Research Articles on SEMA3E
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Product Notes
The SEMA3E sema3e (Catalog #AAA2018304) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Semaphorin 3E (SEMA3E) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the SEMA3E sema3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF- LDAGTYFCQT VEHSIVHTVR KITLEIVEEE RVDEMFSKDY EEEISHKMPC PMQSNIPQVS KPWYKEFLQL IGYSNFQRVE EYCEKVWCTD. It is sometimes possible for the material contained within the vial of "Semaphorin 3E (SEMA3E), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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