ALK-2 / ACVR1 / ALK2 recombinant protein

Recombinant Rat ALK-2 / ACVR1 / ALK2 Protein (Fc tag)

Cat. Num. AAA2547460

• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: ALK-2 / ACVR1 / ALK2; Recombinant Rat ALK-2 / ACVR1 / ALK2 Protein (Fc tag); ACVR1; Acvrlk2; ALK-2 / ACVR1 / ALK2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 509

• Application Notes: The recombinant rat ACVR1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 344 amino acids and has a predicted molecular mass of 38.5 kDa. The apparent molecular mass of the protein is approximately 43 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met 21
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ALK-2 / ACVR1 / ALK2 recombinant protein

Background: ALK-2, also termed as ACVR1, was initially identified as an activin type I receptor because of its ability to bind activin in concert with ActRII or ActRIIB. ALK-2 is also identified as a BMP type I receptor. It has been demonstrated that ALK-2 forms complex with either the BMP-2/7-bound BMPR-II or ACVR2A /ACVR2B. ALK-1 and ALK-2 presenting in the yeast Saccharomyces cerevisiae are two haspin homologues. Both ALK-1 and ALK-2 exhibit a weak auto-kinase activity in vitro, and are phosphoproteins in vivo. ALK-1 and ALK-2 levels peak in mitosis and late-S/G2. Control of protein stability plays a major role in ALK-2 regulation. The half-life of ALK-2 is particularly short in G1. Overexpression of ALK-2, but not of ALK-1, causes a mitotic arrest, which is correlated to the kinase activity of the protein. This suggests a role for ALK-2 in the control of mitosis. Endoglin is phosphorylated on cytosolic domain threonine residues by the TGF-beta type I receptors ALK-2 and ALK-5 in prostate cancer cells. Endoglin did not inhibit cell migration in the presence of constitutively active ALK-2. Defects in ALK-2 are a cause of fibrodysplasia ossificans progressiva (FOP).

Description: A DNA sequence encoding the rat ACVR1 (P80201) (Met1-Glu123) was expressed, fused with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 13324702
• NCBI GeneID: 79558
• NCBI Accession #: NP_077812.1
• NCBI GenBank Nucleotide #: NM_024486.1
• UniProt Accession #: P80201
• Molecular Weight: 57,195 Da
• NCBI Official Full Name: activin receptor type-1
• NCBI Official Synonym Full Names: activin A receptor type 1
• NCBI Official Symbol: Acvr1
• NCBI Protein Information: activin receptor type-1
• UniProt Protein Name: Activin receptor type-1
• UniProt Gene Name: Acvr1
• UniProt Synonym Gene Names: Acvrlk2; ACTR-I; SKR1; TSR-I

NCBI Description

potential transmembrane protein serine kinase that acts as an activin receptor [RGD, Feb 2006]

Uniprot Description

ALK2: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin. May be involved for left-right pattern formation during embryogenesis. Interacts with FKBP1A. Interacts with FCHO1. Expressed in normal parenchymal cells, endothelial cells, fibroblasts and tumor-derived epithelial cells. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.

Protein type: EC 2.7.11.30; Kinase, protein; Membrane protein, integral; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; STKR family; TKL group; Type1 subfamily

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: activin receptor complex; apical part of cell; integral to plasma membrane

Molecular Function: activin binding; activin receptor activity, type I; ATP binding; growth factor binding; protein homodimerization activity; protein kinase activity; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity; SMAD binding; transforming growth factor beta binding; transforming growth factor beta receptor activity, type I; transmembrane receptor protein serine/threonine kinase activity

Biological Process: activin receptor signaling pathway; acute inflammatory response; BMP signaling pathway; determination of left/right symmetry; G1/S transition of mitotic cell cycle; gastrulation; gastrulation with mouth forming second; germ cell development; heart development; in utero embryonic development; mesoderm development; mesoderm formation; negative regulation of activin receptor signaling pathway; negative regulation of signal transduction; neural crest cell migration; patterning of blood vessels; peptidyl-threonine phosphorylation; pharyngeal system development; positive regulation of bone mineralization; positive regulation of cell migration; positive regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of ossification; regulation of skeletal muscle development; smooth muscle cell differentiation; transforming growth factor beta receptor signaling pathway; urogenital system development