Human fibroblast activation protein, alpha ELISA Kit | FAP elisa kit

Human Seprase, FAP ELISA Kit

Cat. Num. AAA937486

• Reactivity: Human
• Synonyms: fibroblast activation protein; alpha; Human Seprase; FAP ELISA Kit; Human Seprase (FAP) ELISA kit; DKFZp686G13158; DPPIV; FAPA; alpha subunit; integral membrane serine protease; seprase; FAP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human FAM3C. No significant cross-reactivity or interference between human FAM3C and analogues was observed.
• Sequence Length: 761

• Samples: Serum, Plasma, Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5 pg/ml-4000 pg/ml
• Sensitivity: 15.6 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FAP elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FAM3C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FAM3C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FAM3C is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FAM3C bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6679749
• NCBI GeneID: 14089
• NCBI Accession #: NP_032012.1
• NCBI GenBank Nucleotide #: NM_007986.3
• UniProt Accession #: P97321
• Molecular Weight: 87,945 Da
• NCBI Official Full Name: seprase
• NCBI Official Synonym Full Names: fibroblast activation protein
• NCBI Official Symbol: Fap
• NCBI Protein Information: seprase; integral membrane serine protease; fibroblast activation protein alpha
• UniProt Protein Name: Seprase
• Protein Family: Prolyl endopeptidase
• UniProt Gene Name: Fap
• UniProt Entry Name: SEPR_MOUSE

NCBI Description

This gene belongs to the serine protease family. The encoded protein is an inducible cell-surface bound glycoprotein specifically expressed in tumor-associated fibroblasts and pericytes of epithelial tumors and has protease and gelatinase activity. The protein plays a role in remodeling of the extracellular matrix (ECM) and may affect tumorigenesis and tissue repair. Alternately spliced transcript variants of this gene are described in the literature (PMID 9139873), but the full-length sequence of these variants is not available. [provided by RefSeq, Apr 2013]

Uniprot Description

FAP: In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. Belongs to the peptidase S9B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.14.5; Membrane protein, integral; Protease; EC 3.4.21.26

Cellular Component: extracellular space; cell surface; cell projection; focal adhesion; membrane; apical part of cell; lamellipodium; plasma membrane; integral to membrane; extracellular region; cell junction

Molecular Function: integrin binding; peptidase activity; protein homodimerization activity; protease binding; hydrolase activity; dipeptidyl-peptidase activity; serine-type peptidase activity; serine-type endopeptidase activity; endopeptidase activity

Biological Process: proteolysis involved in cellular protein catabolic process; apoptosis; endothelial cell migration; angiogenesis; cell adhesion; proteolysis