Rat insulin-like growth factor 1 (somatomedin C) ELISA Kit | IGF1 elisa kit

Rat Insulin-like growth factor 1, IGF-1 ELISA Kit

Cat. Num. AAA700632

• Gene Names: IGF1; IGF-IB
• Reactivity: Rat
• Synonyms: insulin-like growth factor 1 (somatomedin C); Rat Insulin-like growth factor 1; IGF-1 ELISA Kit; IGF1A; IGFI; OTTHUMP00000195084; insulin-like growth factor 1; IGF-1; IGF1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of monkey IGF-1. No significant cross-reactivity or interference between monkey IGF-1 and analogues was observed.
• Sequence Length: 143

• Samples: Serum, Plasma
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5 ng/ml-4000 ng/ml
• Sensitivity: 15.6 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGF1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGF-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGF-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGF-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGF-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 126722801
• NCBI GeneID: 100008668
• NCBI Accession #: NP_001075495.1
• NCBI GenBank Nucleotide #: NM_001082026.1
• UniProt Accession #: Q95222; O18846
• Molecular Weight: 16,092 Da
• NCBI Official Full Name: insulin-like growth factor I
• NCBI Official Symbol: IGF1
• NCBI Official Synonym Symbols: IGF-IB
• NCBI Protein Information: insulin-like growth factor I; IGF-I; somatomedin; insulin-like growth factor IB
• UniProt Protein Name: Insulin-like growth factor I
• Protein Family: Insulin-like growth factor
• UniProt Gene Name: IGF1
• UniProt Synonym Gene Names: IGF-1; IGF-I
• UniProt Entry Name: IGF1_RABIT

Uniprot Description

Function: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation