FAM3B recombinant protein

FAM3B Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120795

• Gene Names: FAM3B; 2-21; ORF9; PANDER; PRED44; C21orf11; C21orf76
• Purity: >95% as determined by SDS-PAGE
• Synonyms: FAM3B; FAM3B Protein; Human; Recombinant (hFc Tag); Human FAM3B Protein (Fc Tag); 42056 Protein; C21orf11 Protein; C21orf76 Protein; ORF9 Protein; PANDER Protein; PRED44 Protein; family with sequence similarity 3; member B; FAM3B recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser235

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 30
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human FAM3B isoform B (P58499-1)(Met 1-Ser 235) was fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for FAM3B recombinant protein

Background: Pancreatic derived factor, also known as FAM3B, is an islet-specific secreted cytokine specifically expressed at high levels in the islets of Langerhans of the endocrine pancreas. FAM3B protein is present in alpha- and beta- cells of pancreatic islets, insulin-secreting beta-TC3 cells, and glucagon-secreting alpha-TC cells. FAM3B causes apoptosis of beta-cells as assessed by electron microscopy, annexin I¤ fluorescent staining, and flow-cytometric terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay. FAM3B activated caspase-3 while not affect cytosolic Ca²⁺ levels or nitric oxide levels. Hense, FAM3B may have a role in the process of pancreatic-cell apoptosis of primary islet and cell lines. FAM3B secretion is regulated by glucose and other insulin secretagogues. This islet-specific secreted cytokine is secreted from both pancreatic alpha- and beta- cells. Glucose stimulates FAM3B secretion dose dependently in beta- cell lines and primary islets but not in alpha-cells. It is likely cosecreted with insulin via the same regulatory mechanisms and structure and conformation is vital for FAM3B secretion.

References

Cao X, et al. (2003) Pancreatic-derived factor (FAM3B), a novel islet cytokine, induces apoptosis of insulin-secreting beta-cells. Diabetes. 52(9): 2296-303.Yang J, et al. (2005) Mechanisms of glucose-induced secretion of pancreatic-derived factor (PANDER or FAM3B) in pancreatic beta-cells. Diabetes. 54(11): 3217-28.Xu W, et al. (2005) Interferon-gamma-induced regulation of the pancreatic derived cytokine FAM3B in islets and insulin-secreting betaTC3 cells. Mol Cell Endocrinol. 240(1-2): 74-81.

NCBI and Uniprot Product Information

• NCBI GI #: 46255032
• NCBI GeneID: 54097
• NCBI Accession #: NP_996847.1
• NCBI GenBank Nucleotide #: NM_206964.1
• UniProt Accession #: P58499
• Molecular Weight: 25,982 Da
• NCBI Official Full Name: protein FAM3B isoform b
• NCBI Official Synonym Full Names: family with sequence similarity 3, member B
• NCBI Official Symbol: FAM3B
• NCBI Official Synonym Symbols: 2-21; ORF9; PANDER; PRED44; C21orf11; C21orf76
• NCBI Protein Information: protein FAM3B; D21M16SJHU19e; pancreatic derived factor; pancreatic-derived factor; cytokine-like protein 2-21
• UniProt Protein Name: Protein FAM3B
• Protein Family: Protein
• UniProt Gene Name: FAM3B
• UniProt Synonym Gene Names: C21orf11; C21orf76; PANDER
• UniProt Entry Name: FAM3B_HUMAN

Uniprot Description

FAM3B: Induces apoptosis of alpha and beta cells in a dose- and time-dependent manner. Present in insulin secretory granules and likely cosecreted with insulin. Belongs to the FAM3 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Nuclear envelope; Secreted, signal peptide; Apoptosis; Cytokine

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: extracellular space; extracellular region

Molecular Function: cytokine activity

Biological Process: insulin secretion; apoptosis