Immunohistochemistry (IHC)
(MBS9607788 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit KLK5 Polyclonal Antibody | anti-KLK5 antibody
KLK5 Antibody
Gene Names
KLK5; SCTE; KLKL2; KLK-L2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
KLK5; Polyclonal Antibody; KLK5 Antibody; HK5; Kallikrein 5; Kallikrein L2; Kallikrein like 2; Kallikrein like protein 2; Kallikrein related peptidase 5; Kallikrein-5; Kallikrein-like protein 2; Kallikrein5; KLK 5; KLK L2; KLK-L2; KLK5_HUMAN; KLKL 2; KLKL2; SCTE; Stratum corneum tryptic enzyme; UNQ570/PRO1132; anti-KLK5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
KLK5 antibody detects endogenous levels of total KLK5
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
293
Applicable Applications for anti-KLK5 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:100-1:200
IHC: 1:100-1:200
Immunogen
A synthetic peptide of human KLK5
Subcellular Location
Secreted.
Tissue Specificity
Expressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (80%), Bovine (90%), Horse (80%), Sheep (90%), Rabbit (100%), Dog (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9607788 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9607788 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts of Mouse brain tissue lysate, using KLK5antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts of Mouse brain tissue lysate, using KLK5antibody. The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-KLK5 antibody
Description: Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its expression is up-regulated by estrogens and progestins. The encoded protein is secreted and may be involved in desquamation in the epidermis. Alternative splicing results in multiple transcript variants encoding the same protein.
Function: May be involved in desquamation.
Subunit Structure: Interacts with SPINK9.
Similarity: Belongs to the peptidase S1 family. Kallikrein subfamily.
Function: May be involved in desquamation.
Subunit Structure: Interacts with SPINK9.
Similarity: Belongs to the peptidase S1 family. Kallikrein subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 32 kDa
Predicted: 33 kDa
Predicted: 33 kDa
NCBI Official Full Name
kallikrein-5 preproprotein
NCBI Official Synonym Full Names
kallikrein related peptidase 5
NCBI Official Symbol
KLK5
NCBI Official Synonym Symbols
SCTE; KLKL2; KLK-L2
NCBI Protein Information
kallikrein-5
UniProt Protein Name
Kallikrein-5
Protein Family
UniProt Gene Name
KLK5
UniProt Synonym Gene Names
SCTEImportedManual assertion inferred from database entriesiHGNC:6366; KLK-L2
NCBI Description
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its expression is up-regulated by estrogens and progestins. The encoded protein is secreted and may be involved in desquamation in the epidermis. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Uniprot Description
May be involved in desquamation.
Research Articles on KLK5
Similar Products
Product Notes
The KLK5 klk5 (Catalog #AAA9607788) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KLK5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KLK5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:100-1:200. Researchers should empirically determine the suitability of the KLK5 klk5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLK5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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