Mouse Fatty acid-binding protein, heart ELISA Kit | Fabp3 elisa kit

Mouse Fatty acid-binding protein, heart ELISA Kit

Cat. Num. AAA761454

• Gene Names: FABP3; MDGI; FABP11; H-FABP; M-FABP; O-FABP
• Reactivity: Mouse
• Synonyms: Fatty acid-binding protein; heart; Mouse Fatty acid-binding protein; heart ELISA Kit; Fabp3/FABP3/H-FABP/MDGI/FABP11/fatty acid binding protein 11/fatty acid binding protein 3; muscle and heart (mammary-derived growthinhibitor)/Fatty acid-binding protein 3/Fatty acid-binding protein 3; muscle/fatty acid-binding protein; heart/Heart-type fatty acid-binding protein/H-FABPM-FABP/Mammary-derived growth inhibitor/MDGI/Muscle fatty acid-binding protein/O-FABP; Fabp3 elisa kit

• Reactivity: Mouse
• Sequence Length: 133

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Fabp3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Fabp3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Fabp3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Fabp3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Fabp3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4758328
• NCBI GeneID: 2170
• NCBI Accession #: NP_004093.1
• NCBI GenBank Nucleotide #: NM_004102.3
• Molecular Weight: 14,858 Da
• NCBI Official Full Name: fatty acid-binding protein, heart
• NCBI Official Synonym Full Names: fatty acid binding protein 3
• NCBI Official Symbol: FABP3
• NCBI Official Synonym Symbols: MDGI; FABP11; H-FABP; M-FABP; O-FABP
• NCBI Protein Information: fatty acid-binding protein, heart
• UniProt Protein Name: Fatty acid-binding protein, heart
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: FABP3
• UniProt Synonym Gene Names: FABP11; MDGI; H-FABP; MDGI; M-FABP
• UniProt Entry Name: FABPH_HUMAN

NCBI Description

The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

FABP3: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 1p33-p32

Cellular Component: cytosol; extracellular space; sarcoplasm

Molecular Function: cytoskeletal protein binding; icosatetraenoic acid binding; long-chain fatty acid transporter activity; protein binding

Biological Process: cholesterol homeostasis; fatty acid metabolic process; negative regulation of cell proliferation; phospholipid homeostasis; regulation of fatty acid oxidation; response to drug; response to insulin stimulus; triacylglycerol catabolic process