Typical Standard Curve/Testing Data
Canine Fatty Acid Binding Protein 3, Muscle And Heart ELISA Kit | FABP3 elisa kit
Canine Fatty Acid Binding Protein 3, Muscle And Heart ELISA Kit
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Principle of the Assay: FABP3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FABP3 antibody and an FABP3-HRP conjugate. The assay sample and buffer are incubated together with FABP3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FABP3 concentration since FABP3 from samples and FABP3-HRP conjugate compete for the anti-FABP3 antibody binding site. Since the number of sites is limited, as more sites are occupied by FABP3 from the sample, fewer sites are left to bind FABP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FABP3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer. [provided by RefSeq, Jul 2008]
Uniprot Description
FABP3: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
Protein type: Lipid-binding
Chromosomal Location of Human Ortholog: 1p33-p32
Cellular Component: extracellular space; cytosol; sarcoplasm
Molecular Function: protein binding; icosatetraenoic acid binding; cytoskeletal protein binding; long-chain fatty acid transporter activity
Biological Process: response to drug; negative regulation of cell proliferation; cholesterol homeostasis; regulation of fatty acid oxidation; fatty acid metabolic process; phospholipid homeostasis; response to insulin stimulus
Research Articles on FABP3
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Product Notes
The Canine FABP3 fabp3 (Catalog #AAA739933) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA739933 ELISA Kit recognizes Canine FABP3. It is sometimes possible for the material contained within the vial of "Fatty Acid Binding Protein 3, Muscle And Heart, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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