Mouse coagulation factor V, FV ELISA Kit | FV elisa kit

Mouse coagulation factor V, FV ELISA Kit

Cat. Num. AAA706255

• Gene Names: F5; Cf5; Cf-5; AI173222
• Reactivity: Mouse
• Synonyms: coagulation factor V; FV; Mouse coagulation factor V; FV ELISA Kit; FVL; PCCF; activated protein c cofactor; coagulation factor V jinjiang A2 domain; factor V Leiden; FV elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse F V. No significant cross-reactivity or interference between mouse F V and analogues was observed.
• Sequence Length: 2183

• Samples: serum, plasma
• Assay Type: Quantitative Sandwich
• Detection Range: 78 ng/ml -5000 ng/ml
• Sensitivity: <19.5 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FV elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FV has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FV present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FV is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FV bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6679731
• NCBI GeneID: 14067
• NCBI Accession #: NP_032002.1
• NCBI GenBank Nucleotide #: NM_007976.2
• UniProt Accession #: O88783; Q3UTQ2; Q3UV80
• Molecular Weight: 247,230 Da
• NCBI Official Full Name: coagulation factor V
• NCBI Official Synonym Full Names: coagulation factor V
• NCBI Official Symbol: F5
• NCBI Official Synonym Symbols: Cf5; Cf-5; AI173222
• NCBI Protein Information: coagulation factor V; activated protein C cofactor
• UniProt Protein Name: Coagulation factor V
• UniProt Gene Name: F5
• UniProt Entry Name: FA5_MOUSE

NCBI Description

This gene encodes a glycoprotein coagulation factor that plays a critical role in the process of blood coagulation and hemostasis. The encoded protein is activated by thrombin, to generate a heterodimer containing heavy and light chains held together by calcium ions. About half of the mice lacking the encoded protein die at an embryonic stage possible due to abnormal yolk-sac vasculature while the remaining animals succumbed to massive hemorrhage immediately after birth. A point mutation in this gene has been shown to cause disseminated intravascular thrombosis in the perinatal period, resulting in frequent deaths of newborn mice. [provided by RefSeq, Apr 2015]

Uniprot Description

Function: Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.

Enzyme regulation: Inhibited by SERPINA5