Factor X ELISA Pair Set | F10 elisa pair set

Factor X Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118347

• Synonyms: Factor X; Factor X Matched ELISA Antibody Pair Set; Human; Human Coagulation Factor X/F10 ELISA Pair Set; coagulation factor X; coagulation factor 10 Matched ELISA Antibody Pair Set; coagulation factor X Matched ELISA Antibody Pair Set; FX Matched ELISA Antibody Pair Set; FXA Matched ELISA Antibody Pair Set; F10 elisa pair set

• Specificity: Quantitative determination of Human Factor X

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 5.47-350pg/mL
• Sensitivity: 5.47pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of mouse anti-Human Coagulation Factor X/F10 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human Coagulation Factor X/F10 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 30 ng of recombinant Human Coagulation Factor X/F10. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 350 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for F10 elisa pair set

Coagulation factor X, also known as FX, F10, Eponym Stuart-Prower factor, and thrombokinase, is an enzyme of the coagulation cascade. It is one of the vitamin K-dependent serine proteases, and plays a crucial role in the coagulation cascade and blood clotting, as the first enzyme in the common pathway of thrombus formation. Factor X deficiency is one of the rarest of the inherited coagulation disorders. FX deficiency among the most severe of the rare coagulation defects, typically including hemarthroses, hematomas, and umbilical cord, gastrointestinal, and central nervous system bleeding. Factor X is synthesized in the liver as a mature heterodimer formed from a single-chain precursor, and vitamin K is essential for its synthesis. Factor X is activated into factor Xa (FXa) by both factor IX (with its cofactor, factor VIII in a complex known as intrinsic Xase) and factor VII (with its cofactor, tissue factor in a complex known as extrinsic Xase) through cleaving the activation propeptide. As the first member of the final common pathway or thrombin pathway, FXa converts prothrombin to thrombin in the presence of factor Va, Ca2+, and phospholipid during blood clotting and cleaves prothrombin in two places (an arg-thr and then an arg-ile bond). This process is optimized when factor Xa is complexed with activated cofactor V in the prothrombinase complex. Inborn deficiency of factor X is very uncommon, and may present with epistaxis (nose bleeds), hemarthrosis (bleeding into joints) and gastrointestinal blood loss. Apart from congenital deficiency, low factor X levels may occur occasionally in a number of disease states. Furthermore, factor X deficiency may be seen in amyloidosis, where factor X is adsorbed to the amyloid fibrils in the vasculature.

This Factor X Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human Factor X. It contains Human Factor X capture antibody, Human Factor X detector antibody (HRP) and a highly purified Baculovirus-Insect-expressed recombinant Human Factor X protein.

Product Categories/Family for F10 elisa pair set

Serine Proteases and Regulators

References

Rosen ED. (2002) Gene targeting in hemostasis. Factor X. Front Biosci. 7: d1915-25.Uprichard J, et al. (2002) Factor X deficiency. Blood Rev. 16(2): 97-110.Borensztajn K, et al. (2008) Factor Xa: at the crossroads between coagulation and signaling in physiology and disease. Trends Mol Med. 14(10): 429-40.Menegatti M, et al. (2009) Factor X deficiency. Semin Thromb Hemost. 35(4): 407-15.