Surfactant Associated Protein C (SPC) Recombinant Protein | SPC recombinant protein

Recombinant Surfactant Associated Protein C (SPC)

Cat. Num. AAA2011534

• Gene Names: Sftpc; SP-C
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Surfactant Associated Protein C (SPC); Recombinant Surfactant Associated Protein C (SPC); SPC recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- FSIGST GIVLYDYQRL LTAYKPAPGT YCYIMKMAPE SIPSLEALAR KFKNFQAKSS TPTSKLGQEE GHSAGSDSDS SGRDLAFLGL AVSTLCGELP LYYI
• Sequence Length: 194

• Applicable Applications for SPC recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Phe95~Ile194 (Accession # P11685) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 8394341
• NCBI GeneID: 50683
• NCBI Accession #: NP_059038.1
• NCBI GenBank Nucleotide #: NM_017342.2
• UniProt Accession #: P11685; Q52MA6
• Molecular Weight: 12.3kDa
• NCBI Official Full Name: pulmonary surfactant-associated protein C
• NCBI Official Synonym Full Names: surfactant protein C
• NCBI Official Symbol: Sftpc
• NCBI Official Synonym Symbols: SP-C
• NCBI Protein Information: pulmonary surfactant-associated protein C; surfactant associated protein C; Surfactant pulmonary-associated protein C; Surfactant, pulmonary-associated protein C; pulmonary surfactant-associated proteolipid SPL(Val)
• UniProt Protein Name: Pulmonary surfactant-associated protein C
• UniProt Gene Name: Sftpc
• UniProt Synonym Gene Names: Sftp2; SP-C
• UniProt Entry Name: PSPC_RAT

NCBI Description

promotes reduction of surface tension in the lung [RGD, Feb 2006]

Uniprot Description

Function: Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.

Subcellular location: Secreted › extracellular space › surface film.

Miscellaneous: Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).

Sequence similarities: Contains 1 BRICHOS domain.