Western Blot (WB)
(Fig.1. Western blot of specific proteins fraction using MyBioSource Nuclear and Cytoplasmic Protein Extraction Kit. Sample: 293T cells lysate. C: cytoplasmic extraction was analyzed using alpha-tubulin antibody (A01080). N: nuclear extraction was analyzed using alpha-tubulin antibody (A01080).)
Nuclear and Cytoplasmic Protein Extraction Kit
Nuclear and Cytoplasmic Protein Extraction Kit
Synonyms
Nuclear and Cytoplasmic Protein; Nuclear and Cytoplasmic Protein Extraction Kit; Nuclear and Cytoplasmic Protein extraction kit
Preparation and Storage
Store at 2-8 degree C.
Western Blot (WB)
(Fig.1. Western blot of specific proteins fraction using MyBioSource Nuclear and Cytoplasmic Protein Extraction Kit. Sample: 293T cells lysate. C: cytoplasmic extraction was analyzed using alpha-tubulin antibody (A01080). N: nuclear extraction was analyzed using alpha-tubulin antibody (A01080).)
Western Blot (WB)
(Fig.1. Western blot of specific proteins fraction using MyBioSource Nuclear and Cytoplasmic Protein Extraction Kit. Sample: 293T cells lysate. C: cytoplasmic extraction was analyzed using alpha-tubulin antibody (A01080). N: nuclear extraction was analyzed using alpha-tubulin antibody (A01080).)
Western Blot (WB)
(Fig.2. Western blot of specific proteins fraction using MyBioSource Nuclear and Cytoplasmic Protein Extraction Kit. Sample: 293T cells lysate. C: cytoplasmic extraction was analyzed using Histone H3 antibody (A01070). N: nuclear extraction was analyzed using Histone H3 antibody (A01070). )
Western Blot (WB)
(Fig.2. Western blot of specific proteins fraction using MyBioSource Nuclear and Cytoplasmic Protein Extraction Kit. Sample: 293T cells lysate. C: cytoplasmic extraction was analyzed using Histone H3 antibody (A01070). N: nuclear extraction was analyzed using Histone H3 antibody (A01070). )
Related Product Information for Nuclear and Cytoplasmic Protein extraction kit
Principle of the Assay: The Nuclear and Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of crude cytoplasmic and nuclear extracts from mammalian cultured cells or tissue. This Kit is based on allowing cells to swell with hypotonic buffer. And then the cells are disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer. Non-denatured, active proteins are purified in less than two hours.
Background/Introduction: The preparation of an extract from nuclei is often the first step in studying nuclear proteins and their interactions. The resulting preparation can be used directly in Western blotting, Electrophoresis Mobility Shift Assay (EMSA), footprinting analysis, transcription assays, or as a starting point for the purification of regulatory proteins.
Background/Introduction: The preparation of an extract from nuclei is often the first step in studying nuclear proteins and their interactions. The resulting preparation can be used directly in Western blotting, Electrophoresis Mobility Shift Assay (EMSA), footprinting analysis, transcription assays, or as a starting point for the purification of regulatory proteins.
Similar Products
Product Notes
The Nuclear and Cytoplasmic Protein (Catalog #AAA9718629) is an Extraction Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Nuclear and Cytoplasmic Protein, Extraction Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
