Total Membrane Protein Extraction Kit
Total Membrane Protein Extraction Kit
Synonyms
Total Membrane Protein; Total Membrane Protein Extraction Kit; Total Membrane Protein extraction kit
Preparation and Storage
Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment.
Western Blot (WB)
(Fig. Western blot of specific proteins fraction using MyBioSource Total Membrane Protein Extraction Kit. Sample: 293T cells lysate. H: hydrophilic phase extraction was analyzed using Na+/K+-ATPase Antibody. M: Membrane protein extraction was analyzed using Na+/K+-ATPase Antibody.)
Related Product Information for Total Membrane Protein extraction kit
Principle of the Assay: The Total Membrane Protein Extraction Kit is a simple, rapid and reproducible method to prepare cellular protein fractions highly enriched in membrane and hydrophobic proteins, based on temperature-dependent phase separation. The sample is first mixed and homogenized in the membrane protein extraction buffers. After a brief incubation at 37 degree C, the sample is centrifuged, producing an upper aqueous phase and a lower detergent-rich phase. Proteins that were anchored to the membrane or proteins containing one or two transmembrane domains are efficiently partitioned to the detergent-rich phase.
Background/Introduction: The preparation of an extract from membrane is often the first step in studying membrane proteins and their interactions. The resulting preparation can be used directly in Western blotting, Dot blot, Immunoprecipitation, or as a starting point for the purification of specific proteins.
Background/Introduction: The preparation of an extract from membrane is often the first step in studying membrane proteins and their interactions. The resulting preparation can be used directly in Western blotting, Dot blot, Immunoprecipitation, or as a starting point for the purification of specific proteins.
