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Western Blot (WB) (Western Blot (WB) analysis of TAF II p68 (N380) pAb at 1:500 dilutionLane1:3T3-L1 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug))

Rabbit TAF II Polyclonal Antibody | anti-TAFII antibody

TAF II p68 (N380) Polyclonal Antibody

Gene Names
TAF15; Npl3; RBP56; TAF2N; TAFII68
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
TAF II; Polyclonal Antibody; TAF II p68 (N380) Polyclonal Antibody; TATA-binding protein-associated factor 2N; 68 kDa TATA-binding protein-associated factor; TAF(II)68; TAFII68; RNA-binding protein 56; TAF15; RBP56; TAF2N; anti-TAFII antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
TAF II p68 (N380) polyclonal antibody detects endogenous levels of TAF II protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
589
Applicable Applications for anti-TAFII antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 347-398 of Human TAF II p68.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TAF II p68 (N380) pAb at 1:500 dilutionLane1:3T3-L1 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug))

Western Blot (WB) (Western Blot (WB) analysis of TAF II p68 (N380) pAb at 1:500 dilutionLane1:3T3-L1 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of TAF II p68 (N380) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.)

Immunohistochemistry (IHC) (Immunohistochemistry (IHC) analyzes of TAF II p68 (N380) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.)
Related Product Information for anti-TAFII antibody
TAF II p68, also known as TAF15, RBP56 or TAF2N, is a member of the RNA polymerase II multiprotein complex and is present in certain TFIID subcomplexes. Expressed throughout the body, TAF II p68 is a single stranded RNA binding protein that shares homology with TLS (translocated in liposarcoma) and EWS (Ewing's sarcoma), both of which are proto-oncogenes. Fusion of TAF II p68 with certain transcription factors transforms it into an oncoprotein with oncogenic potential at the N-terminus. When, for example, TAF II p68 is fused with TEC (translocated in extraskeletal chondrosarcoma), the fusion pair acts as an oncoprotein in which TAF II p68 is the transactivation domain. Defects in the gene encoding TAF II p68 are associated with extraskeletal myxoid chondrosarcoma, a malignant soft tumor.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 68kDa
NCBI Official Full Name
TATA-binding protein-associated factor 2N isoform 2
NCBI Official Synonym Full Names
TATA-box binding protein associated factor 15
NCBI Official Symbol
TAF15
NCBI Official Synonym Symbols
Npl3; RBP56; TAF2N; TAFII68
NCBI Protein Information
TATA-binding protein-associated factor 2N
UniProt Protein Name
TATA-binding protein-associated factor 2N
UniProt Gene Name
TAF15
UniProt Synonym Gene Names
RBP56; TAF2N; TAF(II)68; TAFII68
UniProt Entry Name
RBP56_HUMAN

NCBI Description

This gene encodes a member of the TET family of RNA-binding proteins. The encoded protein plays a role in RNA polymerase II gene transcription as a component of a distinct subset of multi-subunit transcription initiation factor TFIID complexes. Translocations involving this gene play a role in acute leukemia and extraskeletal myxoid chondrosarcoma, and mutations in this gene may play a role in amyotrophic lateral sclerosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

TAF15: RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II). A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3. Belongs to the RRM TET family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; Oncoprotein; RNA-binding

Chromosomal Location of Human Ortholog: 17q11.1-q11.2

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; nucleotide binding

Biological Process: positive regulation of transcription, DNA-dependent

Disease: Chondrosarcoma, Extraskeletal Myxoid

Research Articles on TAFII

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Product Notes

The TAFII taf15 (Catalog #AAA3002698) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TAF II p68 (N380) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TAF II can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the TAFII taf15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TAF II, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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