Rat Endothelin 1 ELISA Kit | ET-1 elisa kit

Rat Endothelin 1 ELISA Kit

Cat. Num. AAA765180

• Gene Names: EDN1; ET1; QME; PPET1; ARCND3; HDLCQ7
• Reactivity: Rat
• Synonyms: Endothelin 1; Rat Endothelin 1 ELISA Kit; ET-1/EDN1/ET1/HDLCQ7/PPET1/Preproendothelin-1/endothelin 1/preproendothelin-1; ET-1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of ET-1. No significant cross-reactivity or interference between ET-1 and analogues was observed.
• Sequence Length: 211

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 1.25-80pg/ml
• Sensitivity: 4.688pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ET-1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ET-1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ET-1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ET-1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- ET-1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ET-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ET-1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 269995983
• NCBI GeneID: 1906
• NCBI Accession #: NP_001161791.1
• NCBI GenBank Nucleotide #: NM_001168319.1
• Molecular Weight: 24,425 Da
• NCBI Official Full Name: endothelin-1 isoform 2 preproprotein
• NCBI Official Synonym Full Names: endothelin 1
• NCBI Official Symbol: EDN1
• NCBI Official Synonym Symbols: ET1; QME; PPET1; ARCND3; HDLCQ7
• NCBI Protein Information: endothelin-1
• UniProt Protein Name: Endothelin-1
• UniProt Gene Name: EDN1
• UniProt Synonym Gene Names: PPET1; ET-1
• UniProt Entry Name: EDN1_HUMAN

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

EDN1: Endothelins are endothelium-derived vasoconstrictor peptides. Belongs to the endothelin/sarafotoxin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p24.1

Cellular Component: cytoplasm; cytosol; extracellular region; extracellular space

Molecular Function: cytokine activity; endothelin A receptor binding; endothelin B receptor binding; hormone activity; protein binding

Biological Process: artery smooth muscle contraction; body fluid secretion; calcium-mediated signaling; cartilage development; cell growth; cell surface receptor linked signal transduction; cell-cell signaling; dorsal/ventral pattern formation; elevation of cytosolic calcium ion concentration; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); epithelial fluid transport; G-protein coupled receptor protein signaling pathway; G-protein signaling, phospholipase D activating pathway; glucose transport; heart development; histamine secretion; in utero embryonic development; inositol phosphate-mediated signaling; leukocyte activation; maternal process involved in parturition; membrane depolarization; middle ear morphogenesis; multicellular organismal aging; negative regulation of blood coagulation; negative regulation of cAMP biosynthetic process; negative regulation of cellular protein metabolic process; negative regulation of hormone secretion; negative regulation of nitric-oxide synthase biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; neural crest cell development; neutrophil chemotaxis; nitric oxide transport; patterning of blood vessels; peptide hormone secretion; phosphoinositide 3-kinase cascade; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell size; positive regulation of heart rate; positive regulation of hormone secretion; positive regulation of JNK activity; positive regulation of MAP kinase activity; positive regulation of mitosis; positive regulation of nitric oxide biosynthetic process; positive regulation of odontogenesis; positive regulation of prostaglandin secretion; positive regulation of smooth muscle cell proliferation; positive regulation of smooth muscle contraction; positive regulation of transcription from RNA polymerase II promoter; prostaglandin biosynthetic process; protein kinase C activation; protein kinase C deactivation; regulation of pH; regulation of sensory perception of pain; regulation of systemic arterial blood pressure by endothelin; regulation of vasoconstriction; respiratory gaseous exchange; response to activity; response to amino acid stimulus; response to lipopolysaccharide; response to nicotine; response to ozone; response to testosterone stimulus; rhythmic excitation; sensory perception of pain; superoxide release; vasoconstriction; vein smooth muscle contraction

Disease: Auriculocondylar Syndrome 3; Question Mark Ears, Isolated