Human Endothelin 1 ELISA Kit | EDN1 elisa kit

Human Endothelin 1 ELISA Kit

Cat. Num. AAA824949

• Gene Names: EDN1; ET1; QME; PPET1; ARCND3; HDLCQ7
• Reactivity: Human
• Applications: ELISA
• Synonyms: Endothelin 1; Human Endothelin 1 ELISA Kit; Endothelin-1; Preproendothelin-1; PPET1; EDN1 elisa kit

• Reactivity: Human
• Specificity: The Human CXCL2 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CXCL2 proteins within the range of 15.6 pg/ml-1000 pg/ml.
• Sequence Length: 211

• Applicable Applications for EDN1 elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: 7 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EDN1 elisa kit

Principle of the Assay: The MyBioSource' Human CXCL2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL2 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human CXCL2 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: MIP is a member of the aquaporin family of membrane-bound water channels. MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains. Major intrinsic protein (MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens. The major intrinsic protein (MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water (aquaporins), glycerol and other small molecules in microbial, plant and animal cells.

NCBI and Uniprot Product Information

• NCBI GI #: 269995983
• NCBI GeneID: 1906
• NCBI Accession #: NP_001161791.1
• NCBI GenBank Nucleotide #: NM_001168319.1
• UniProt Accession #: P05305; Q96DA1
• Molecular Weight: 24,425 Da
• NCBI Official Full Name: endothelin-1 isoform 2 preproprotein
• NCBI Official Synonym Full Names: endothelin 1
• NCBI Official Symbol: EDN1
• NCBI Official Synonym Symbols: ET1; QME; PPET1; ARCND3; HDLCQ7
• NCBI Protein Information: endothelin-1; preproendothelin-1
• UniProt Protein Name: Endothelin-1
• Protein Family: Endothelin
• UniProt Gene Name: EDN1
• UniProt Synonym Gene Names: PPET1; ET-1
• UniProt Entry Name: EDN1_HUMAN

NCBI Description

The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

EDN1: Endothelins are endothelium-derived vasoconstrictor peptides. Belongs to the endothelin/sarafotoxin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p24.1

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: protein binding; hormone activity; endothelin B receptor binding; cytokine activity; endothelin A receptor binding

Biological Process: response to nicotine; positive regulation of JNK activity; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of nitric oxide biosynthetic process; regulation of systemic arterial blood pressure by endothelin; heart development; response to lipopolysaccharide; middle ear morphogenesis; sensory perception of pain; prostaglandin biosynthetic process; positive regulation of MAP kinase activity; elevation of cytosolic calcium ion concentration; negative regulation of cAMP biosynthetic process; cell surface receptor linked signal transduction; cell-cell signaling; negative regulation of nitric-oxide synthase biosynthetic process; protein kinase C activation; cell growth; neutrophil chemotaxis; rhythmic excitation; positive regulation of mitosis; negative regulation of blood coagulation; positive regulation of heart rate; response to testosterone stimulus; respiratory gaseous exchange; peptide hormone secretion; response to amino acid stimulus; leukocyte activation; patterning of blood vessels; membrane depolarization; protein kinase C deactivation; regulation of vasoconstriction; positive regulation of transcription from RNA polymerase II promoter; response to activity; superoxide release; positive regulation of odontogenesis; epithelial fluid transport; phosphoinositide 3-kinase cascade; positive regulation of cell size; neural crest cell development; G-protein signaling, phospholipase D activating pathway; positive regulation of smooth muscle cell proliferation; negative regulation of hormone secretion; negative regulation of cellular protein metabolic process; negative regulation of transcription from RNA polymerase II promoter; glucose transport; vein smooth muscle contraction; histamine secretion; nitric oxide transport; positive regulation of cell proliferation; regulation of pH; positive regulation of smooth muscle contraction; artery smooth muscle contraction; vasoconstriction; inositol phosphate-mediated signaling; in utero embryonic development; positive regulation of hormone secretion; calcium-mediated signaling; multicellular organismal aging; body fluid secretion; G-protein coupled receptor protein signaling pathway; dorsal/ventral pattern formation; response to ozone; cartilage development; maternal process involved in parturition; positive regulation of prostaglandin secretion; regulation of sensory perception of pain; positive regulation of cell migration

Disease: Question Mark Ears, Isolated; Auriculocondylar Syndrome 3