Nicotinamide N-Methyltransferase Active Protein | NNMT active protein

Recombinant Human Nicotinamide N-Methyltransferase, Active

Cat. Num. AAA147127

• Purity: Greater than 95.0% as determined by SDS-PAGE
• Synonyms: Nicotinamide N-Methyltransferase; Recombinant Human Nicotinamide N-Methyltransferase; Active; Nicotinamide N-methyltransferase; EC 2.1.1.1; NNMT; NNMT Human; Nicotinamide N-Methyltransferase Human Recombinant; NNMT active protein

• Host: Escherichia Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE
• Form/Format: Sterile filtered colorless solution; Protein solution (1mg/ml) containing 20 mM Tris-HCl buffer (pH 8.0) and 20% glycerol
• Sequence: MGSSHHHHHH SSGLVPRGSH MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC LDGVKGDLLI DIGSGPTIYQ LLSACESFKE IVVTDYSDQN LQELEKWLKK EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG AVPLPPADCV LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE GLFSLVARKL SRPL
• Sequence Length: 264

• Biological Activity: Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at-20 degree C for longer periods of time.; For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Avoid multiple freeze-thaw cycles

Related Product Information for NNMT active protein

Description: NNMT Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 284 amino acids (1-264 a.a) and having a molecular mass of 37.7kDa. NNMT is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction: NNMT is part of the family of transferases, especially those transferring one-carbon group methyltransferases. NNMT is mostly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. NNMT catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is significant for biotransformation of many drugs and xenobiotic compounds. NNMT is accountable for the enzymatic activity which uses S-adenosyl methionine as the methyl donor. NNMT expression is related with tumor stage and DFS time in hepatocellular carcinoma cases. NNMT is a good candidate as a tumor marker of various kinds of cancers. NNMT serum levels have significance in the premature detection and in the management of patients with colorectal cancer.

Product Categories/Family for NNMT active protein

Enzymes; Transferase; NNMT Human; Active

NCBI and Uniprot Product Information

• NCBI GI #: 730163
• NCBI GeneID: 4837
• UniProt Accession #: P40261
• Molecular Weight: 29,574 Da
• NCBI Official Full Name: Nicotinamide N-methyltransferase
• NCBI Official Synonym Full Names: nicotinamide N-methyltransferase
• NCBI Official Symbol: NNMT
• NCBI Protein Information: nicotinamide N-methyltransferase
• UniProt Protein Name: Nicotinamide N-methyltransferase
• Protein Family: Nicotinamide N-methyltransferase
• UniProt Gene Name: NNMT

NCBI Description

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.