ESAM recombinant protein

Human ESAM

Cat. Num. AAA692066

• Gene Names: ESAM; W117m
• Reactivity: Human
• Purity: > 95% by SDS-PAGE & silver stain
• Synonyms: ESAM; Human ESAM; Endothelial cell-selective adhesion molecule; ESAM recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: > 95% by SDS-PAGE & silver stain
• Form/Format: Lyophilized
• Sequence: N-Terminal Sequence: UNDER WORK!
• Sequence Length: 254

• Buffer: PBS
• Preparation and Storage: The lyophilized human ESAM, though stable at room temperature, is best stored desiccated below 0 degree C.

Testing Data

Related Product Information for ESAM recombinant protein

Endothelial cell selective adhesion molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein that belongs to the JAM family of immunoglobulin superfamily molecules. Human ESAM is synthesized as a 390 amino acid (aa) protein composed of a 29 aa signal peptide, a 216 aa extracellular region, a putative 26 aa transmembrane segment, and a 119 aa cytoplasmic domain. The extracellular region contains one V-type and one C2-type Ig domain and is involved in hemophilic adhesion. In the cytoplasmic domain, there is a docking site for the multifunctional adaptor protein MAGI1. The extracellular region of human ESAM shows 90%, 74%, 69% and 67% aa identity with monkey, canine, mouse and rat extracellular ESAM, respectively. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes, and can be found associated with cell to cell junctions. Whether ESAM is restricted to a particular junctional type is not clear. ESAM deficient mice have no defect in vascularization but do have reduced angiogenic potential. This may be due to a decreased migratory response to FGF2. Soluble ESAM is fused to a C-terminal His-tag (6x His).

Product Categories/Family for ESAM recombinant protein

Cytokines & Growth Factors

References

1. Hirata et al, J Biol Chem 276 (2001) 2. Nasdala et al, J Biol Chem 277 (2002) 3. Wegmann et al, Exp Cell Res 300 (2004) 4. Ishida et al, J Biol Chem 278 (2003)

NCBI and Uniprot Product Information

• NCBI GI #: 148664205
• NCBI GeneID: 90952
• NCBI Accession #: NP_620411.2
• NCBI GenBank Nucleotide #: NM_138961.2
• UniProt Accession #: Q96AP7; Q96T50
• Molecular Weight: 27,86 kDa
• NCBI Official Full Name: endothelial cell-selective adhesion molecule
• NCBI Official Synonym Full Names: endothelial cell adhesion molecule
• NCBI Official Symbol: ESAM
• NCBI Official Synonym Symbols: W117m
• NCBI Protein Information: endothelial cell-selective adhesion molecule; 2310008D05Rik; LP4791 protein; HUEL (C4orf1)-interacting protein
• UniProt Protein Name: Endothelial cell-selective adhesion molecule
• Protein Family: Endothelial cell-selective adhesion molecule
• UniProt Gene Name: ESAM
• UniProt Entry Name: ESAM_HUMAN

Uniprot Description

Function: Can mediate aggregation most likely through a homophilic molecular interaction

By similarity.

Subunit structure: Interacts with BAIAP1

By similarity.

Subcellular location: Cell junction › adherens junction

By similarity. Cell junction › tight junction

By similarity. Cell membrane; Single-pass type I membrane protein

By similarity.

Tissue specificity: Highly expressed in endothelial cells. Ref.1

Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.Contains 1 Ig-like V-type (immunoglobulin-like) domain.