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Western Blot (WB)
(Anti-ARID1A rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:242 kDa Observed band size:251 kDa)
Rabbit anti-Human ARID1A Polyclonal Antibody | anti-ARID1A antibody
Anti-ARID1A Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
ARID1A; Polyclonal Antibody; Anti-ARID1A Antibody; Rabbit Polyclonal; ARID1A Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-B120 Antibody; Anti-BAF250 Antibody; Anti-BAF250a Antibody; Anti-BM029 Antibody; Anti-C1orf4 Antibody; Anti-CSS2 Antibody; Anti-ELD Antibody; Anti-hELD Antibody; Anti-hOSA1 Antibody; Anti-MRD14 Antibody; Anti-OSA1 Antibody; Anti-P270 Antibody; Anti-SMARCF1 Antibody; anti-ARID1A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ARID1A
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-ARID1A antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
IHC-P: 1:250-1:1000
ICC/IF: 1:500-1:2500
IHC-P: 1:250-1:1000
ICC/IF: 1:500-1:2500
Immunogen
E Coli-derived Human ARID1A fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human ARID1A fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-ARID1A rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:242 kDa Observed band size:251 kDa)
Western Blot (WB)
(Anti-ARID1A rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:242 kDa Observed band size:251 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human ARID1A in human brain with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human ARID1A in human brain with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human ARID1A in human rectum with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human ARID1A in human rectum with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunofluorescence (IF)
(Immunofluorescence staining of ARID1A in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ARID1A polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of ARID1A in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ARID1A polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Related Product Information for anti-ARID1A antibody
The At-rich interactive domain 1A (ARID1A) is frequently mutated in gastric cancers (GCs) with a poor prognosis. Growing evidence indicates that loss of ARID1A expression leads to activation of the phosphatidylinositol 3-kinase (PI3K)/AKT pathway by AKT phosphorylation. ARID1A upregulates its downstream target CDKN1A and suppresses HCC cell proliferation and migration through inhibiting MVIH, that deficiency or loss of functional mutations of ARID1A in HCC cells might contribute to the increased activity of certain cancer-promoting lncRNAs. ARID1A has a dominant role in maintaining chromatin accessibility at enhancers, while the contribution of ARID1B is evident only in the context of ARID1A mutation. ARID1B knockdown in ARID1A mutant ovarian cancer cells causes similar loss of enhancer architecture, suggesting that this is a conserved function underlying the synthetic lethality between ARID1A and ARID1B. In endometriosis, the down-regulated ofARID1A gene was highly correlated with its promoter hyper-methylation. ROS decreased the expression level of ARID1A gene via regulating methylation of its promoter which contributing to the understanding of the pathogenesis of endometriosis. The possible mechanism of ARID1A gene promoter hyper-methylation is ROS up-regulated DNMT1gene expression. ARID1A promotes tumor initiation in the liver but suppresses metastasis in established tumors. Loss of ARID1A expression enhances radioresistance of pancreatic cancer through activation of PI3K/AKT pathway, which may be a promising target for radiosensitization of ARID1A-deficient pancreatic cancer.
Product Categories/Family for anti-ARID1A antibody
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Product Notes
The ARID1A (Catalog #AAA8109457) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ARID1A Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARID1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500-1:1000 IHC-P: 1:250-1:1000 ICC/IF: 1:500-1:2500. Researchers should empirically determine the suitability of the ARID1A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARID1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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