HER3 / ErbB3 recombinant protein

Recombinant Human HER3 / ErbB3 Protein (Fc tag)

Cat. Num. AAA2546419

• Gene Names: ERBB3; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: HER3 / ErbB3; Recombinant Human HER3 / ErbB3 Protein (Fc tag); ErbB3; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; MGC88033; HER3 / ErbB3 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 1342

• Application Notes: The recombinant human ErbB3/mFc is a disulfide-linked homodimer. The reduced monomer comprises 858 amino acids and has a predicted molecular mass of 95 kDa. The apparent molecular mass of the protein is approximately 119-129 in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ser 20
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for HER3 / ErbB3 recombinant protein

Background: ErbB3, also known as Her3 (human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

Description: A DNA sequence encoding the human ErbB3 (NP_001973.2) (Met1-Thr643)was fused with Fc region of mouse IgG at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 54792100
• NCBI GeneID: 2065
• NCBI Accession #: NP_001973.2
• NCBI GenBank Nucleotide #: NM_001982.3
• UniProt Accession #: P21860; Q9BUD7; A8K6L6; B4DIK7; B4DV32; E9PDT8
• Molecular Weight: 77,426 Da
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-3 isoform 1
• NCBI Official Synonym Full Names: erb-b2 receptor tyrosine kinase 3
• NCBI Official Symbol: ERBB3
• NCBI Official Synonym Symbols: HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-3
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-3
• UniProt Gene Name: ERBB3
• UniProt Synonym Gene Names: HER3

NCBI Description

This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

HER3: a receptor tyrosine kinase of the EGFR family. Binds and is activated by neuregulins and NTAK. Can form homodimers or ErbB-2/ErbB-3 heterodimers. Kinase domain lacks activity but heterodimerizes with other EGFRs to transduce growth signals. May be required for HER2 activity. Elevated expression in breast and other tumors is indicative of poor outcome. A secreted form is expressed in metastatic prostate cancer Two alternatively spliced isoforms have been described.

Protein type: EC 2.7.10.1; EGFR family; Kinase, protein; Membrane protein, integral; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 12q13.2

Cellular Component: basolateral plasma membrane; extracellular space; integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: growth factor binding; identical protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein heterodimerization activity; protein tyrosine kinase activator activity; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; transmembrane receptor activity

Biological Process: cranial nerve development; heart development; MAPKKK cascade; negative regulation of cell adhesion; negative regulation of neuron apoptosis; negative regulation of secretion; negative regulation of signal transduction; neuron apoptosis; peripheral nervous system development; phosphoinositide 3-kinase cascade; phosphoinositide-mediated signaling; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell proliferation; regulation of phosphoinositide 3-kinase cascade; Schwann cell differentiation; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Lethal Congenital Contracture Syndrome 2