Rat Estradiol ELISA Kit | ER elisa kit
Rat Estradiol receptor (ER) ELISA Kit
Principle of the Assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for ESR1 and Horseradish Peroxidase (HRP) conjugated ESR1. The competitive inhibition reaction is launched between with HRP labeled ESR1 and unlabeled ESR1 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of ESR1 in the sample. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
acts as a transcriptional activator when bound to estrogen; may play a role in myocardial regulation [RGD, Feb 2006]
Uniprot Description
ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.
Protein type: DNA-binding; Nuclear receptor
Chromosomal Location of Human Ortholog: 1q11
Cellular Component: cytoplasm; Golgi apparatus; mitochondrion; neuron projection; nuclear chromatin; nucleus; perikaryon; perinuclear region of cytoplasm; plasma membrane; protein complex; T-tubule; terminal bouton
Molecular Function: ATPase binding; beta-catenin binding; chromatin binding; DNA binding; DNA binding transcription factor activity; enzyme binding; estrogen receptor activity; estrogen receptor binding; estrogen response element binding; hormone binding; identical protein binding; ligand-dependent nuclear receptor activity; protein binding; protein complex binding; protein kinase binding; sequence-specific DNA binding; steroid binding; steroid hormone receptor activity; TATA-binding protein binding; transcription factor binding; type 1 metabotropic glutamate receptor binding; zinc ion binding
Biological Process: androgen metabolic process; antral ovarian follicle growth; decidualization; elevation of cytosolic calcium ion concentration; epithelial cell development; intracellular estrogen receptor signaling pathway; male gonad development; negative regulation of glucose import; negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of smooth muscle cell proliferation; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; osteoblast development; phospholipase C-activating G-protein coupled receptor signaling pathway; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of epithelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of nitric oxide biosynthetic process; positive regulation of nitric-oxide synthase activity; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of transcriptional preinitiation complex assembly; regulation of apoptosis; regulation of inflammatory response; regulation of neuron apoptosis; regulation of toll-like receptor signaling pathway; regulation of transcription, DNA-templated; response to estradiol; response to estrogen; Sertoli cell development; Sertoli cell proliferation; stem cell differentiation; steroid hormone mediated signaling; steroid hormone receptor signaling pathway; transcription from RNA polymerase II promoter; transcription, DNA-dependent; uterus development; vagina development
Research Articles on ER
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Product Notes
The Rat ER esr1 (Catalog #AAA285402) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA285402 ELISA Kit recognizes Rat ER. It is sometimes possible for the material contained within the vial of "Estradiol, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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