Rat estrogen receptor 1 ELISA Kit | ESR1 elisa kit

rat Estradiol receptor, ER ELISA Kit

Cat. Num. AAA704037

• Gene Names: Esr1; Esr; ER-alpha; RNESTROR
• Reactivity: Rat
• Synonyms: estrogen receptor 1; rat Estradiol receptor; ER ELISA Kit; RP1-130E4.1; DKFZp686N23123; ER; ESR; ESRA; Era; NR3A1; estrogen receptor alpha; estrogen receptor alpha delta 3*; 4; 5; 6; 7*/819-2 isoform; estrogen receptor alpha delta 4 +49 isoform; estrogen receptor; ESR1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat ESR1. No significant cross-reactivity or interference between Rat ESR1 and analogues was observed.
• Sequence Length: 600

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Detection Range: 31.25 pg/ml-2000 pg/ml
• Sensitivity: 7.81 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Transcription/Transcription regulation
• Protein Biological Process 3: Transcription
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 6978815
• NCBI GeneID: 24890
• NCBI Accession #: NP_036821.1
• NCBI GenBank Nucleotide #: NM_012689.1
• UniProt Accession #: P06211
• Molecular Weight: 67,030 Da
• NCBI Official Full Name: estrogen receptor
• NCBI Official Synonym Full Names: estrogen receptor 1
• NCBI Official Symbol: Esr1
• NCBI Official Synonym Symbols: Esr; ER-alpha; RNESTROR
• NCBI Protein Information: estrogen receptor; Er alpha; estradiol receptor; estrogen receptor protein; estrogen receptor 1 (alpha); estrogen receptor alpha variant delta 4; nuclear receptor subfamily 3 group A member 1; estrogen receptor alpha c-terminus splice variant 1-2
• UniProt Protein Name: Estrogen receptor
• Protein Family: Estrogen receptor
• UniProt Gene Name: Esr1
• UniProt Synonym Gene Names: Esr; Estr; Nr3a1; ER
• UniProt Entry Name: ESR1_RAT

NCBI Description

acts as a transcriptional activator when bound to estrogen; may play a role in myocardial regulation [RGD, Feb 2006]

Uniprot Description

Function: Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades