CD38 active protein

CD38 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122602

• Gene Names: Cd38; ADPRC 1; Cd38-rs1
• Purity: >97% as determined by SDS-PAGE
• Synonyms: CD38; CD38 Protein; Mouse; Recombinant (His Tag); Mouse CD38 Protein (His Tag); ADPRC 1-rs1 Protein; ADPRC1 Protein; Cd38 Protein; Cd38-rs1 Protein; I-19 Protein; CD38 molecule; CD38 active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM MES, 0.15M NaCl, pH6.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Leu45-Thr304

• Species: Mouse
• Activity: Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDPribose. The specific activity is >50,000pmols/min/ug.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 48
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse CD38 (NP_031672.2)(Leu 45-Thr 304) was expressed, fused with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD38 active protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 38 (CD38), also known as ADP-ribosyl cyclase, is a glycoprotein found on the surface of many immune cells (white blood cells), including CD4⁺, CD8⁺, B and natural killer cells. It shares several characteristics with ADP-ribosyl cyclase 2 CD157. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD⁺ to ADP-ribose. It also functions in cell adhesion, signal transduction and calcium signaling. CD38 has been used as a prognostic marker in leukemia. It can also be used to identify plasma cells.

Product Categories/Family for CD38 active protein

Cancer Drug Targets

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.

NCBI and Uniprot Product Information

• NCBI GI #: 31542362
• NCBI GeneID: 12494
• NCBI Accession #: NP_031672.2
• NCBI GenBank Nucleotide #: NM_007646.4
• UniProt Accession #: P56528; Q8BFY8
• Molecular Weight: 34,408 Da
• NCBI Official Full Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• NCBI Official Synonym Full Names: CD38 antigen
• NCBI Official Symbol: Cd38
• NCBI Official Synonym Symbols: ADPRC 1; Cd38-rs1
• NCBI Protein Information: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; I-19; NIM-R5 antigen; cADPr hydrolase 1
• UniProt Protein Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• Protein Family: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase
• UniProt Gene Name: Cd38
• UniProt Synonym Gene Names: ADPRC 1; cADPr hydrolase 1
• UniProt Entry Name: CD38_MOUSE

NCBI Description

This gene encodes a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Knockout mice deficient for this gene display altered humoral immune responses. In addition, knockout mice exhibit higher locomotor activity and defects in nurturing and social behaviors. [provided by RefSeq, Sep 2015]

Uniprot Description

CD38: Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. Belongs to the ADP-ribosyl cyclase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Hydrolase; EC 3.2.2.6; EC 2.4.99.20; Cell surface; Cell cycle regulation; Apoptosis; Membrane protein, integral; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Cellular Component: cell surface; membrane; intracellular membrane-bound organelle; integral to membrane; plasma membrane; cytosol; nucleus

Molecular Function: transferase activity; phosphorus-oxygen lyase activity; hydrolase activity; hydrolase activity, acting on glycosyl bonds; NAD+ nucleosidase activity; NAD(P)+ nucleosidase activity

Biological Process: response to drug; metabolic process; positive regulation of transcription, DNA-dependent; response to hormone stimulus; positive regulation of insulin secretion; positive regulation of cell growth; B cell receptor signaling pathway; elevation of cytosolic calcium ion concentration; response to hydroperoxide; response to hypoxia; positive regulation of B cell proliferation; positive regulation of vasoconstriction; negative regulation of transcription, DNA-dependent; negative regulation of bone resorption; negative regulation of apoptosis