EphA7 / EHK3 recombinant protein

Recombinant Rat EphA7 / EHK3 Protein (His tag)

Cat. Num. AAA2547463

• Gene Names: EPHA7; EHK3; EK11; EHK-3; HEK11
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: EphA7 / EHK3; Recombinant Rat EphA7 / EHK3 Protein (His tag); EPHA7; Ehk-3; Ehk3; EphA7 / EHK3 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 279

• Application Notes: The recombinant rat EPHA7 comprises 523 amino acids and predicts a molecular mass of 58.9 kDa. The apparent molecular mass of the recombinant protein is approximately 59 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Gln 28
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for EphA7 / EHK3 recombinant protein

Background: Ephrin type -A receptor 7, also known as EphA7, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Eph receptor-mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. Down-regulation of EphA7 secondary to hypermethylation has been reported in colorectal cancer. The expression of EphA7 was reduced in all tested gastric cancer cell lines; however, there is marked variability in expression among gastric carcinoma specimens. EphA7 may have roles in the pathogenesis and development of gastric carcinomas.

Description: A DNA sequence encoding the rat EPHA7 (Met1-Ser539) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 568599818
• NCBI GeneID: 2045
• NCBI Accession #: NP_001275559.1
• NCBI GenBank Nucleotide #: NM_001288630.1
• UniProt Accession #: Q15375; Q59G40; Q5VTU0; Q8N368; Q9H124; A0AUX7; B2R8W1; B7ZLJ9; B7ZLK0
• Molecular Weight: 50,521 Da
• NCBI Official Full Name: ephrin type-A receptor 7 isoform 3
• NCBI Official Synonym Full Names: EPH receptor A7
• NCBI Official Symbol: EPHA7
• NCBI Official Synonym Symbols: EHK3; EK11; EHK-3; HEK11
• NCBI Protein Information: ephrin type-A receptor 7
• UniProt Protein Name: Ephrin type-A receptor 7
• UniProt Gene Name: EPHA7
• UniProt Synonym Gene Names: EHK3; HEK11; EHK-3; EK11; hEK11

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Increased expression of this gene is associated with multiple forms of carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

EphA7: a widely-expressed receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A2, -A3, -A4 and -A5. Contains 1 sterile alpha motif (SAM) and two fibronectin type III domains.

Protein type: EC 2.7.10.1; Eph family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 6q16.1

Cellular Component: plasma membrane

Molecular Function: axon guidance receptor activity; chemorepellent activity; GPI-linked ephrin receptor activity; protein binding; protein-tyrosine kinase activity

Biological Process: brain development; branching morphogenesis of a nerve; ephrin receptor signaling pathway; negative chemotaxis; phosphorylation; positive regulation of neuron apoptosis; regulation of caspase activity; regulation of cell-cell adhesion; regulation of peptidyl-tyrosine phosphorylation; regulation of protein amino acid autophosphorylation