Proteinase K Solution 20mg/ml Enzyme
Proteinase K Solution 20mg/ml
Applications
Isolation of high molecular weight DNAIsolation of plasmid and genomic DNA
Isolation of RNA
Inactivation of RNase and DNase activities
Synonyms
Proteinase K Solution 20mg/ml; Proteinase K Solution 20mg/ml enzyme
Form/Format
Supplied as a liquid in 10mM Tris HCl, pH7.5, 10mM calcium acetate, 40% glycerol.
Concentration
20mg/ml (varies by lot)
Applicable Applications for Proteinase K Solution 20mg/ml enzyme
Isolation of high molecular weight DNA
Isolation of plasmid and genomic DNA
Isolation of RNA
Inactivation of RNase and DNase activities
Isolation of plasmid and genomic DNA
Isolation of RNA
Inactivation of RNase and DNase activities
Application Notes
The recommended working concentration for Proteinase K is 0.05-1mg/ml. Use 3ul of a 20mg/ml solution per 1.5ml of bacterial culture. Optimal dilutions to be determined by researcher.
Grade
Molecular Biology Grade
Source
Tritirachium album limber
Nomenclature
EC 3.4.21.14
Solubility
Colorless, clear, complete
pH
7.5
Activity
>= 35.5 units/mg
Unit Definition
One unit liberates 1umole of Folin positive amino acids, measured as Tyrosine, at 37°C, pH 7.5, using urea-denatured hemoglobin as the substrate.
CAS No
39450-01-6
Advantages
- Ready to use, provided in liquid form containing 40% glycerol.
- Smaller contamination risk (liquid form).
- Can be stored at -20°C still having the enzyme in liquid form.
- Excellent stability.
- Smaller contamination risk (liquid form).
- Can be stored at -20°C still having the enzyme in liquid form.
- Excellent stability.
Notes
- Proteinase K is not inactivated bymetal-chelating agents like EDTA, bythiol-reactive reagents (such asmonoiodoacetic acid,p-chloromercuribenzoate) or by specifictrypsin and chymotrypsin inhibitors. Phenylmethylsulfonyl fluoride and diisopropyl phosphorofluoridate completely inhibit the enzyme.
- Proteinase K is stable over a wide pHrange: 4-12.5.- Raising the temperature of the reactionfrom 37°C to 50°C-65°C can increase the activity of Proteinase K.
- Proteinase K can be inactivated at 95°C for 10 minutes (some enzymatic activity may still remain)
- Proteinase K retains its activity in thepresence of SDS (0.5-1%) or urea(1-4M).
- Depending on the application the enzyme is used in the absence orpresence of 0.2-1% SDS or 1-4M urea.
- Proteinase K is stable over a wide pHrange: 4-12.5.
- Proteinase K can be inactivated at 95°C for 10 minutes (some enzymatic activity may still remain)
- Proteinase K retains its activity in thepresence of SDS (0.5-1%) or urea(1-4M).
- Depending on the application the enzyme is used in the absence orpresence of 0.2-1% SDS or 1-4M urea.
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Proteinase K Solution 20mg/ml enzyme
Proteinase K is classified as a non-specific serine protease. It is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic orhydrophobic amino acids.
Product Categories/Family for Proteinase K Solution 20mg/ml enzyme
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Product Notes
The Proteinase K Solution 20mg/ml (Catalog #AAA653959) is an Enzyme and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Proteinase K Solution 20mg/ml can be used in a range of immunoassay formats including, but not limited to, Isolation of high molecular weight DNA Isolation of plasmid and genomic DNA Isolation of RNA Inactivation of RNase and DNase activities. The recommended working concentration for Proteinase K is 0.05-1mg/ml. Use 3ul of a 20mg/ml solution per 1.5ml of bacterial culture. Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the Proteinase K Solution 20mg/ml for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Proteinase K Solution 20mg/ml, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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