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Testing Data (Sequence of amino acids of C2277-06J1.)

CD26, His-tag, Hi-5 Active Protein

CD26, Recombinant, Human, His-tag, Hi-5 (DPP IV, Dipeptidyl Peptidase IV, DPPIV, ADABP, ADCP2, TP103)

Purity
Highly Purified
95% by SDS PAGE
Synonyms
CD26; His-tag; Hi-5; Recombinant; Human; Hi-5 (DPP IV; Dipeptidyl Peptidase IV; DPPIV; ADABP; ADCP2; TP103); Hi-5 active protein
Ordering
For Research Use Only!
Host
Hi-5 cell
Purity/Purification
Highly Purified
95% by SDS PAGE
Form/Format
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 100mM sodium chloride, 1mM EDTA, 10% glycerol.
Application Notes
Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling.
Biological Activity
50units/mg
Unit Definition
One unit will hydrolyze 1umole of p-nitroaniline per minute at pH 8.0, at 37 degree C using 1mM of Gly-Pro p-nitroanilde as a substrate.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Sequence of amino acids of C2277-06J1.)

Testing Data (Sequence of amino acids of C2277-06J1.)

SDS-Page

(10% SDS-PAGE using 2ug of C2277-06J1.)

SDS-Page (10% SDS-PAGE using 2ug of C2277-06J1.)

Testing Data

(Optical density was measured at 405nm after incubating enzyme solution with 1mM of p-nitroanilid as a substrate.)

Testing Data (Optical density was measured at 405nm after incubating enzyme solution with 1mM of p-nitroanilid as a substrate.)
Related Product Information for CD26, His-tag, Hi-5 active protein
D26 is a complex enzyme expressed on the surface of most cell types and is a serine exopeptidase that cleaves x-proline dipeptides from the N-terminus of polypeptides. DPP4 protein is associated with intracellular signal transduction, apoptosis and plays an important role in tumor biology. There are at least 63 substrates which can bind specifically to DPP4 enzyme including growth factors, chemokines, neuro peptides. Furthermore, DPP4 plays a major role in glucose metabolism by cleaving incretins such as glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1).

Recombinant protein corresponding to aa39-766 of human CD26, with a C-terminal His-tag, expressed in high-5 cells using baculovirus expression system.
Product Categories/Family for CD26, His-tag, Hi-5 active protein

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Product Notes

The CD26, His-tag, Hi-5 (Catalog #AAA635645) is an Active Protein produced from Hi-5 cell and is intended for research purposes only. The product is available for immediate purchase. Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling. Researchers should empirically determine the suitability of the CD26, His-tag, Hi-5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD26, His-tag, Hi-5, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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