Rat C-Peptide ELISA Kit | C-Peptide elisa kit
Rat C-Peptide ELISA Kit
Reactivity
Rat
Synonyms
C-Peptide; Rat C-Peptide ELISA Kit; C-Peptide elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat C-peptide. No significant cross-reactivity or interference between rat C-peptide and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Competitive
Detection Range
1.25 ng/ml-20 ng/ml
Sensitivity
< 1.25 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for C-Peptide elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-peptide. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated C-peptide. A competitive inhibition reaction is launched between C-peptide (Standards or samples) and Biotin-conjugated C-peptide with the pre-coated antibody specific for C-peptide. The more amount of C-peptide in samples, the less antibody bound by Biotin-conjugated C-peptide. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of C-peptide in the sample. The color development is stopped and the intensity of the color is measured.
