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Calibration Curve
(According to the absorbance A275 for the vertical axis, the different concentrations of tyrosine as the horizontal axis, the standard curve was drawn. Then, the enzyme activity was 30U/mg by calculating (Fig.1). Unit definition: One unit is defined as the amount of enzyme that catalyzes the formation of 1 umol of tyrosine per minute at pH 7.5 at 37°C)
Proteinase K (PROK) Enzyme | PROK enzyme
Proteinase K (PROK) Recombinant Protein
Applications
Immunohistochemistry
Purity
90% ± 5% by SDS-PAGE
Synonyms
Proteinase K (PROK); Proteinase K (PROK) Recombinant Protein; PROK enzyme
Host
Recombination
Purity/Purification
90% ± 5% by SDS-PAGE
Form/Format
Liquid, dissolved in Tris-HCl, pH 7.5, 10%glycerol?0.02%NaN3
Applicable Applications for PROK enzyme
Genomic DNA Purification Kit, RNA Purification Kit, Gene Diagnostic Kit, Immunohistochemistry (IHC), ISH
Valid Point
2-8°C for 12 months, -20°C for 18 months
Preparation and Storage
Aliquot and store at<= -20°C. Avoid repeated freeze / thaw cycles
Calibration Curve
(According to the absorbance A275 for the vertical axis, the different concentrations of tyrosine as the horizontal axis, the standard curve was drawn. Then, the enzyme activity was 30U/mg by calculating (Fig.1). Unit definition: One unit is defined as the amount of enzyme that catalyzes the formation of 1 umol of tyrosine per minute at pH 7.5 at 37°C)
Calibration Curve
(According to the absorbance A275 for the vertical axis, the different concentrations of tyrosine as the horizontal axis, the standard curve was drawn. Then, the enzyme activity was 30U/mg by calculating (Fig.1). Unit definition: One unit is defined as the amount of enzyme that catalyzes the formation of 1 umol of tyrosine per minute at pH 7.5 at 37°C)
Protein Purity
(The purity and the molecular weight of MBS7053447 proteinase K were over 95% and 28.9 kDa by SDS-PAGE, respectively (Fig.2).)
Protein Purity
(The purity and the molecular weight of MBS7053447 proteinase K were over 95% and 28.9 kDa by SDS-PAGE, respectively (Fig.2).)
Detection of Nucleic Acid Residue
(By agarose gel electrophoresis to detect nucleic acid, figure 3 shows MBS7053447produced proteinase K without RNA, DNA residues (Fig.3).)
Detection of Nucleic Acid Residue
(By agarose gel electrophoresis to detect nucleic acid, figure 3 shows MBS7053447produced proteinase K without RNA, DNA residues (Fig.3).)
Detection of DNAse Residue
(DNAses remained in lyophilized powder were detected by agarose gel electrophoresis , figure 4 shows MBS7053447 produced proteinase K without DNAse residue after 6h of incubation at 37°C (Fig.4).)
Detection of DNAse Residue
(DNAses remained in lyophilized powder were detected by agarose gel electrophoresis , figure 4 shows MBS7053447 produced proteinase K without DNAse residue after 6h of incubation at 37°C (Fig.4).)
Detection of RNAse Residue
(Agarose gel electrophoresis was performed to check whether there was residual RNase. Figure 5 shows MBS7053447 produced proteinase K without RNAse residue after 16h of incubated at 25°C (Fig.5).)
Detection of RNAse Residue
(Agarose gel electrophoresis was performed to check whether there was residual RNase. Figure 5 shows MBS7053447 produced proteinase K without RNAse residue after 16h of incubated at 25°C (Fig.5).)
Related Product Information for PROK enzyme
Proteinase K is an extracellular serine endoproteinase produced by Tritirachium album Limber. Proteinase K belongs to the peptidase family S8. Its predominant cleavage site is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked ?-amino groups. Proteinase K is a broad-spectrum protease that functions at wide pH and temperature ranges. The optimum pH and temperature for the activity of this protease were approximately pH 7.5-8.0 and 55°C, respectively. In addition, 0.2-1% sodium dodecyl sulfate (SDS) and 10mM carbamide can activate its activity.
The combination of these unique features makes Proteinase K as an important tool in scientific research. Proteinase K can be used in the preparation of biological macromolecules by degrading the constitutive proteins of cells and removing nucleases such as DNases and RNases for RT-PCR. In nucleic acid vaccine and molecular identification, it is also widely used for degrading proteinaceous impurities during the isolation of nucleic acids from biological samples.
The latest launch of high quality of Proteinase K shows its high purity and high enzyme activity by biochemical platform testing. Through repeated inspection, all the data show our company produced Proteinase K without RNA, DNA, RNAse and DNAse residues.
The combination of these unique features makes Proteinase K as an important tool in scientific research. Proteinase K can be used in the preparation of biological macromolecules by degrading the constitutive proteins of cells and removing nucleases such as DNases and RNases for RT-PCR. In nucleic acid vaccine and molecular identification, it is also widely used for degrading proteinaceous impurities during the isolation of nucleic acids from biological samples.
The latest launch of high quality of Proteinase K shows its high purity and high enzyme activity by biochemical platform testing. Through repeated inspection, all the data show our company produced Proteinase K without RNA, DNA, RNAse and DNAse residues.
Product Categories/Family for PROK enzyme
NCBI and Uniprot Product Information
UniProt Accession #
NCBI Official Full Name
proteinase K
UniProt Protein Name
Proteinase K
Protein Family
UniProt Gene Name
PROK
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Product Notes
The PROK prok (Catalog #AAA7053447) is an Enzyme produced from Recombination and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Proteinase K (PROK) can be used in a range of immunoassay formats including, but not limited to, Genomic DNA Purification Kit, RNA Purification Kit, Gene Diagnostic Kit, Immunohistochemistry (IHC), ISH. Researchers should empirically determine the suitability of the PROK prok for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Proteinase K (PROK), Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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