DARPP32 Enzyme | PPP1R1B enzyme

DARPP32 (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B) Blocking Peptide

Cat. Num. AAA653653

• Gene Names: PPP1R1B; DARPP32; DARPP-32
• Applications: Immunohistochemistry
• Purity: Highly Purified; Highly Purified
• Synonyms: DARPP32; DARPP32 (Dopamine and cAMP-regulated Phosphoprotein; Protein phosphatase 1 regulatory subunit 1B; PP1R1B) Blocking Peptide; PPP1R1B enzyme

• Purity/Purification: Highly Purified; Highly Purified
• Form/Format: Supplied as a liquid in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol.

• Applicable Applications for PPP1R1B enzyme: Immunohistochemistry (IHC)
• Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks DARPP-32 Rabbit mAb signal in immunohistochemistry.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse brain using D1075-08 DARPP-32 (19A3) Rabbit mAb D1075-08 in the presence of control peptide (left) or DARPP-32 Blocking Peptide (right).)

Related Product Information for PPP1R1B enzyme

Peptide is used to block DARPP-32 Rabbit mAb reactivity in immunohistochemistry protocols.

DARPP-32 (dopamine and cyclic AMPregulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/ threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological and behavioral phenotype (3).

Product Categories/Family for PPP1R1B enzyme

Molecular Biology; MB-Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 21717548
• NCBI GeneID: 84152
• UniProt Accession #: Q9UD71; Q547V9; Q547W0; Q9H7G1
• Molecular Weight: 22,963 Da
• NCBI Official Full Name: dopamine and cAMP-regulated neuronal phosphoprotein
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory (inhibitor) subunit 1B
• NCBI Official Symbol: PPP1R1B
• NCBI Official Synonym Symbols: DARPP32; DARPP-32
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 1B; dopamine and cAMP-regulated neuronal phosphoprotein 32
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 1B
• Protein Family: Protein phosphatase 1 regulatory
• UniProt Gene Name: PPP1R1B
• UniProt Synonym Gene Names: DARPP32
• UniProt Entry Name: PPR1B_HUMAN

NCBI Description

This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

Function: Inhibitor of protein-phosphatase 1.

Subcellular location: Cytoplasm.

Post-translational modification: Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.Phosphorylation of Thr-34 is required for activity

By similarity.

Sequence similarities: Belongs to the protein phosphatase inhibitor 1 family.