NTPDase 2 / ENTPD2 recombinant protein

Recombinant Human NTPDase 2 / ENTPD2 Protein (aa 29-460, His tag)

Cat. Num. AAA2545684

• Gene Names: ENTPD2; CD39L1; NTPDase-2
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: NTPDase 2 / ENTPD2; Recombinant Human NTPDase 2 / ENTPD2 Protein (aa 29-460; His tag); ENTPD2; CD39L1; NTPDase 2 / ENTPD2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
• Sequence Length: 472

• Application Notes: The recombinant human human ENTPD2 consists of 448 amino acids and predicts a molecular mass of 49.3 KDa. It migrates as an approximately 59 KDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: His
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for NTPDase 2 / ENTPD2 recombinant protein

Background: NTPDase 2, also known as ENTPD2, belongs to the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). Members of E-NTPDase family are nucleotidases able to hydrolyze 5-nucleoside tri- and/or diphosphates; the main role of these enzymes is the termination of purinergic signaling. NTPDases are ubiquitous and were previously shown in other parasites including the trypanosomatides of genus Leishmania and in T. brucei. NTPase activity would act as a timer and is crucial to T. gondii infection. In L. pneumophila it was demonstrated that an E-NTPDase, similar to CD39, is essential for intracellular bacterial multiplication. NTPDase 2 is an integral membrane protein. In the nervous system, it could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Alternative splicing of NTPDase 2 gene results in multiple transcript variants.

Description: A DNA sequence encoding the mature form of human ENTPD2 (Q9Y5L3) (Thr29-Asp460) was expressed, with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4557421
• NCBI GeneID: 954
• NCBI Accession #: NP_001237.1
• NCBI GenBank Nucleotide #: NM_001246.3
• UniProt Accession #: Q9Y5L3; O15464; Q5SPY6; Q5SPY7
• Molecular Weight: 48,535 Da
• NCBI Official Full Name: ectonucleoside triphosphate diphosphohydrolase 2 isoform 2
• NCBI Official Synonym Full Names: ectonucleoside triphosphate diphosphohydrolase 2
• NCBI Official Symbol: ENTPD2
• NCBI Official Synonym Symbols: CD39L1; NTPDase-2
• NCBI Protein Information: ectonucleoside triphosphate diphosphohydrolase 2
• UniProt Protein Name: Ectonucleoside triphosphate diphosphohydrolase 2
• UniProt Gene Name: ENTPD2
• UniProt Synonym Gene Names: CD39L1; NTPDase 2; Ecto-ATPDase 2; Ecto-ATPase 2

NCBI Description

The protein encoded by this gene is the type 2 enzyme of the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). E-NTPDases are a family of ecto-nucleosidases that hydrolyze 5'-triphosphates. This ecto-ATPase is an integral membrane protein. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

ENTPD2: In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Hydrolyzes ADP only to a marginal extent. The order of activity with different substrates is ATP > GTP > CTP = ITP > UTP >> ADP = UDP. Belongs to the GDA1/CD39 NTPase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.1.-; Extracellular matrix; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 9q34.3