Typical Testing Data/Standard Curve (for reference only)
Bovine Sorbitol dehydrogenase, SDH ELISA Kit | SDH elisa kit
Bovine sorbitol dehydrogenase, SDH ELISA Kit
Gene Names
SORD; SORD1; HEL-S-95n
Reactivity
Bovine
Synonyms
Sorbitol dehydrogenase; SDH; Bovine sorbitol dehydrogenase; SDH ELISA Kit; SDH elisa kit
Reactivity
Bovine
Sequence Length
357
Samples
Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.05ng/ml-20ng/ml
Sensitivity
0.017ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SDH elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Bovine Somatotropin (also known as GH1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine GH1 antibody. GH1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine GH1 Antibody is added and binds to GH1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GH1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine GH1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine GH1 antibody. GH1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine GH1 Antibody is added and binds to GH1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GH1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine GH1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,812 Da
NCBI Official Full Name
sorbitol dehydrogenase
NCBI Official Synonym Full Names
sorbitol dehydrogenase
NCBI Official Symbol
SORD
NCBI Official Synonym Symbols
SORD1; HEL-S-95n
NCBI Protein Information
sorbitol dehydrogenase
UniProt Protein Name
Sorbitol dehydrogenase
Protein Family
UniProt Gene Name
SORD
NCBI Description
Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]
Uniprot Description
Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm.
Research Articles on SDH
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Product Notes
The Bovine SDH sord (Catalog #AAA166128) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA166128 ELISA Kit recognizes Bovine SDH. It is sometimes possible for the material contained within the vial of "Sorbitol dehydrogenase, SDH, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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