Gene Sequencing (Extract)
Histatin 1 (HTN1) Recombinant Protein | HTN1 recombinant protein
Recombinant Histatin 1 (HTN1)
Gene Names
HTN1; HIS1
Applications
SDS-Page, Western Blot
Purity
> 95%
Synonyms
Histatin 1 (HTN1); Recombinant Histatin 1 (HTN1); HTN1 recombinant protein
Host
E.coli
Purity/Purification
> 95%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 50ug/mL (varies by lot)
Sequence
D SHEKRHHGYR RKFHEKHHSH REFPFYGDYG SNYLYDN
Applicable Applications for HTN1 recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
Asp20~Asn57
Tags
N-terminal His Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
6.4
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in ddH2O to a concentration of 0-0.05mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Gene Sequencing (Extract)
Gene Sequencing (Extract)
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
N/APredicted Molecular Mass: 11.5kDa
Accurate Molecular Mass: 15kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 15kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
histatin-1
NCBI Official Synonym Full Names
histatin 1
NCBI Official Symbol
HTN1
NCBI Official Synonym Symbols
HIS1
NCBI Protein Information
histatin-1; PPB; post-PB protein; histidine-rich protein 1
UniProt Protein Name
Histatin-1
Protein Family
UniProt Gene Name
HTN1
UniProt Synonym Gene Names
HIS1; PPB; His1 31/57; His1 12/38
UniProt Entry Name
HIS1_HUMAN
NCBI Description
This gene encodes a member of the histatin family of small, histidine-rich, cationic proteins. They function as antimicrobial peptides and are important components of the innate immune system. Histatins are found in saliva and exhibit antibacterial, antifungal activities and function in wound healing. [provided by RefSeq, Aug 2014]
Uniprot Description
HTN1: Histatins are salivary proteins that are considered to be major precursors of the protective proteinaceous structure on tooth surfaces (enamel pellicle). In addition, histatins exhibit antibacterial and antifungal activities. Belongs to the histatin/statherin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 4q13
Cellular Component: extracellular region
Molecular Function: protein binding
Biological Process: killing of cells of another organism; biomineral formation; defense response to bacterium; defense response to fungus
Research Articles on HTN1
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Product Notes
The HTN1 htn1 (Catalog #AAA2009491) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Histatin 1 (HTN1) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). Researchers should empirically determine the suitability of the HTN1 htn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: D SHEKRHHGYR RKFHEKHHSH REFPFYGDYG SNYLYDN. It is sometimes possible for the material contained within the vial of "Histatin 1 (HTN1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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