Enolase-Phosphatase-1 Recombinant Protein | ENOPH1 recombinant protein
Recombinant Human Enolase-Phosphatase-1
Gene Names
ENOPH1; E1; MASA; mtnC; MST145
Purity
Greater than 90.0% as determined by SDS-PAGE.
Synonyms
Enolase-Phosphatase-1; Recombinant Human Enolase-Phosphatase-1; ENOPH1 Human; Enolase-Phosphatase-1 Human Recombinant; Enolase-phosphatase E1; 2; 3-diketo-5-methylthio-1-phosphopentane phosphatase; MASA homolog; ENOPH1; MASA; E1; MST145; FLJ12594; DKFZp586M0524; ENOPH1 recombinant protein
Host
E Coli
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE.
Form/Format
The ENOPH1 solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 100mM NaCl.
Sterile Filtered colorless solution.
Sterile Filtered colorless solution.
Sequence
MGSSHHHHHH SSGLVPRGSH MVVLSVPAEV TVILLDIEGT TTPIAFVKDI LFPYIEENVK EYLQTHWEEE ECQQDVSLLR KQAEEDAHLD GAVPIPAASG NGVDDLQQMI QAVVDNVCWQ MSLDRKTTAL KQLQGHMWRA AFTAGRMKAE FFADVVPAVR KWREAGMKVY IYSSGSVEAQ KLLFGHSTEG DILELVDGHF DTKIGHKVES ESYRKIADSI GCSTNNILFL TDVTREASAA EEADVHVAVV VRPGNAGLTD DEKTYYSLIT SFSELYLPSS T
Sequence Length
173
Related Product Information for ENOPH1 recombinant protein
Description: ENOPH1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 281 amino acids (1-261 a.a.) and having a molecular mass of 31kDa. The ENOPH1 is purified by proprietary chromatographic techniques.
Introduction: Enolase-phosphatase E1 (ENOPH1) belongs to the MasA family of the HAD (halo-acid dehalogenase)-like hydrolase superfamily. ENOPH1 is a bifunctional enzyme which demonstrates both phosphatase and atypical enolase activities. ENOPH1 has a significant role in the ubiquitous methionine salvage pathway which is a biochemical pathway found in all organisms that regulate methionine levels in the cell.
Introduction: Enolase-phosphatase E1 (ENOPH1) belongs to the MasA family of the HAD (halo-acid dehalogenase)-like hydrolase superfamily. ENOPH1 is a bifunctional enzyme which demonstrates both phosphatase and atypical enolase activities. ENOPH1 has a significant role in the ubiquitous methionine salvage pathway which is a biochemical pathway found in all organisms that regulate methionine levels in the cell.
Product Categories/Family for ENOPH1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12,552 Da
NCBI Official Full Name
enolase-phosphatase E1 isoform 2
NCBI Official Synonym Full Names
enolase-phosphatase 1
NCBI Official Symbol
ENOPH1
NCBI Official Synonym Symbols
E1; MASA; mtnC; MST145
NCBI Protein Information
enolase-phosphatase E1; 2,3-diketo-5-methylthio-1-phosphopentane phosphatase; ENOPH1; acireductone synthase
UniProt Protein Name
Enolase-phosphatase E1
UniProt Gene Name
ENOPH1
UniProt Entry Name
ENOPH_HUMAN
Uniprot Description
ENOPH1: Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK- MTPene). Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.3.77; Phosphatase (non-protein); Amino Acid Metabolism - cysteine and methionine
Chromosomal Location of Human Ortholog: 4q21.22
Cellular Component: nucleus; cytosol
Molecular Function: acireductone synthase activity; magnesium ion binding
Biological Process: dephosphorylation; methionine salvage; sulfur amino acid metabolic process; polyamine metabolic process
Research Articles on ENOPH1
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Product Notes
The ENOPH1 enoph1 (Catalog #AAA144671) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MGSSHHH HHH SSGLVPRGSH MVVLSVPAEV TVILLDIEGT TTPIAFVKDI LFPYIEENVK EYLQTHWEEE ECQQDVSLLR KQAEEDAHLD GAVPIPAASG NGVDDLQQMI QAVVDNVCWQ MSLDRKTTAL KQLQGHMWRA AFTAGRMKAE FFADVVPAVR KWREAGMKVY IYSSGSVEAQ KLLFGHSTEG DILELVDGHF DTKIGHKVES ESYRKIADSI GCSTNNILFL TDVTREASAA EEADVHVAVV VRPGNAGLTD DEKTYYSLIT SFSELYLPSS T. It is sometimes possible for the material contained within the vial of "Enolase-Phosphatase-1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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