Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphatidate phosphatase LPIN1 (LPIN1) Standard Curve.)
Human Phosphatidate Phosphatase LPIN1 (LPIN1) ELISA Kit | LPIN1 elisa kit
Human Phosphatidate Phosphatase LPIN1 (LPIN1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphatidate phosphatase LPIN1 (LPIN1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphatidate phosphatase LPIN1 (LPIN1) Standard Curve.)
Background: Lipin-1 has phosphatidate phosphatase activity.This gene represents a candidate gene for human lipodystrophy, characterized by loss of body fat, fatty liver, hypertriglyceridemia, and insulin resistance. Mouse studies suggest that this gene functions during normal adipose tissue development and may also play a role in human triglyceride metabolism. Database analysis identified the human LPIN1 gene, as well as the human and mouse LPIN2 and LPIN3 genes. Consistent with the observed reduction of adipose tissue mass in fld mice, wildtype Lpin1 mRNA was expressed at high levels in adipose tissue and was induced during differentiation of 3T3-L2 preadipocytes. The results indicated that lipin is required for normal adipose tissue development, and provided a candidate gene for human lipodystrophy.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol. Expression of this gene is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolism. Mutations in this gene are associated with metabolic syndrome, type 2 diabetes, acute recurrent rhabdomyolysis, and autosomal recessive acute recurrent myoglobinuria (ARARM). This gene is also a candidate for several human lipodystrophy syndromes. [provided by RefSeq, Mar 2017]
Uniprot Description
LPIN1: Plays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression. Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol. Interacts (via LXXIL motif) with PPARA. Interacts with PPARGC1A. Interaction with PPARA and PPARGC1A leads to the formation of a complex that modulates gene transcription. Interacts with MEF2C. Specifically expressed in skeletal muscle. Also abundant in adipose tissue. Lower levels in some portions of the digestive tract. Inhibited by N-ethylmaleimide. Belongs to the lipin family.
Protein type: EC 3.1.3.4; Phosphatase, lipid
Chromosomal Location of Human Ortholog: 2p25.1
Cellular Component: nucleoplasm; endoplasmic reticulum membrane; mitochondrial outer membrane; transcription factor complex; nuclear membrane; cytoplasm; nuclear envelope; cytosol; nucleus
Molecular Function: peroxisome proliferator activated receptor binding; phosphatidate phosphatase activity; histone deacetylase binding; transcription coactivator activity
Biological Process: mitochondrial fission; organ regeneration; transcription, DNA-dependent; regulation of fat cell differentiation; phosphatidylethanolamine biosynthetic process; glycerophospholipid biosynthetic process; mitotic nuclear envelope disassembly; triacylglycerol biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; fatty acid catabolic process; positive regulation of histone deacetylation; cellular response to insulin stimulus; dephosphorylation; phospholipid metabolic process; actin cytoskeleton reorganization; phosphatidylcholine biosynthetic process; ruffle organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; triacylglycerol mobilization
Disease: Myoglobinuria, Acute Recurrent, Autosomal Recessive
Research Articles on LPIN1
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Product Notes
The Human LPIN1 lpin1 (Catalog #AAA9715452) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715452 ELISA Kit recognizes Human LPIN1. It is sometimes possible for the material contained within the vial of "Phosphatidate Phosphatase LPIN1 (LPIN1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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