IL17RD elisa pair set

IL17RD Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118264

• Synonyms: IL17RD; IL17RD Matched ELISA Antibody Pair Set; Human; Human IL17RD ELISA Pair Set; interleukin 17 receptor D; HH18 Matched ELISA Antibody Pair Set; IL-17RD Matched ELISA Antibody Pair Set; IL17RLM Matched ELISA Antibody Pair Set; SEF Matched ELISA Antibody Pair Set; IL17RD elisa pair set

• Specificity: Quantitative determination of Human IL17RD

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 78.13-5000pg/mL
• Sensitivity: 78.125 pg/ml
• Materials Provided: 1. Capture Antibody 1.0 mg/mL of rabbit anti-IL17RD monoclonal antibody. Dilute to a working concentration of 2.0ug/mL in CBS before coating. ; 2. Detection Antibody 0.5 mg/mL rabbit anti-IL17RD polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 100 ng of recombinant IL17RD. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5000 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for IL17RD elisa pair set

Interleukin-17 receptor D (IL-17D) also known as Interleukin-17 receptor-like protein, is a member of the interleukine-17 receptor family. IL-17RD functions as a feedback inhibitor of fibroblast growth factor-mediated Ras-MAPK signaling and ERK activation. It may inhibit FGF-induced FGFR1 tyrosine phosphorylation, regulate the nuclear ERK signaling pathway by spatially blocking nuclear translocation of activated ERK By similarity, and mediate JNK activation and may be involved in apoptosis. IL-17RD is found expressed in the neopallial cortex, rhombic lip, and dorsal regions of the myelencephalon and the frontal nasal process. IL-17RD is also expressed in the commissural plate and septal area of the forebrain and the hippocampus, lens, and optic cup. In the oral region, IL-17RD is expressed in the tongue and the mesenchyme of the first branchial arch. It is also expressed in the developing inner ear. IL-17RD interacts with both IL-17R-Myc and IL-17RB-Myc. Both the intracellular and extracellular domains of IL-17RD interact with IL-17R. IL-17R forms a heteromeric complex with IL-17RD. Experiment results indicate that IL-17RD can affect IL-17R localization, suggesting that these two molecules are colocalized and associate with each other within cells. The fact that IL-17RD Delta ICD is unable to mediate IL-17 signaling but functions as a dominant-negative form indicates that the intracellular domain of IL-17RD is pivotal. Also, IL-17RD interacts with the IL-17R downstream molecule TRAF6. It has been proposed that the IL-17RD intracellular domain interacts with IL-17R and TRAF6 to deliver the downstream signal.

This IL17RD Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human IL17RD. It contains Human IL17RD capture antibody, Human IL17RD detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human IL17RD protein.

References

Weaver CT, et al.. (2007) IL-17 family cytokines and the expanding diversity of effector T cell lineages. Annu Rev Immunol. 25: 821-52.Rong Z, et al.. (2009) IL-17RD (Sef or IL-17RLM) interacts with IL-17 receptor and mediates IL-17 signaling. Cell Res. 19(2): 208-15.Gaffen SL. (2009) Structure and signalling in the IL-17 receptor family. Nat Rev Immunol. 9(8): 556-67.