IL17RD recombinant protein

Recombinant Human IL17RD Protein (His tag)

Cat. Num. AAA2546216

• Gene Names: IL17RD; SEF; HH18; IL-17RD; IL17RLM
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: IL17RD; Recombinant Human IL17RD Protein (His tag); DKFZp434N1928; FLJ35755; IL-17RD; IL17RLM; MGC133309; SEF; IL17RD recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween80
• Sequence Length: 739

• Application Notes: The secreted recombinant human IL17RD comprises 294 amino acids with a predicted molecular mass of 33.5 kDa. As a result of glycosylation, the apparent molecular mass of rhIL17RD is approximately 55-60 kDa in SDS-PAGE under reducing conditions.
• Source: Human
• Predicted N Terminal: Cys 17
• Protein Construction: A DNA sequence encoding the extracellular domain of human IL17RD (NP_000416.1) (Met 1-Glu 1120) was fused with a polyhistidine tag at the C-terminus.
• Moleclar Mass: The secreted recombinant human IL17RD comprises 294 amino acids with a predicted molecular mass of 33.5 kDa. As a result of glycosylation, the apparent molecular mass of rhIL17RD is approximately 55-60 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method
• Reconstitution: It is recommended that sterile water (200ul) be added to the vial to prepare a stock solution of 0.25 mg/ml.; Concentration is measured by UV-Vis.
• Preparation and Storage: Store under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for IL17RD recombinant protein

Interleukin-17 receptor D (IL-17D) also known as Interleukin-17 receptor-like protein, is a member of interleukine-17 recepter family. IL-17RD functions as a feedback inhibitor of fibroblast growth factor mediated Ras-MAPK signaling and ERK activation. It may inhibit FGF-induced FGFR1 tyrosine phosphorylation, regulate the nuclear ERK signaling pathway by spatially blocking nuclear translocation of activated ERK By similarity, and mediate JNK activation and may be involved in apoptosis. IL-17RD is found expressed in the neopallial cortex, rhombic lip and dorsal regions of the myelencephalon and in the frontal nasal process. IL-17RD is also expressed in the commissural plate and septal area of the forebrain and in the hippocampus, lens and optic cup. In the oral region, IL-17RD is expressed in the tongue and in the mesenchyme of the first branchial arch. It is also expressed in the developing inner ear. IL-17RD interacts with both IL-17R-Myc and IL-17RB-Myc. Both the intracellular and extracellular domains of IL-17RD interact with IL-17R. IL-17R forms a heteromeric complex with IL-17RD. Experiment results indicate that IL-17RD is able to affect IL-17R localization, suggesting that these two molecules are colocalized and associate with each other within cells. The fact that IL-17RD Delta ICD is unable to mediate IL-17 signaling but functions as a dominant-negative form indicates that the intracellular domain of IL-17RD is pivotal. In addition, IL-17RD interacts with the IL-17R downstream molecule TRAF6. It has been proposed that the IL-17RD intracellular domain interacts with IL-17R and TRAF6 to deliver the downstream signal.

NCBI and Uniprot Product Information

• NCBI GI #: 166063983
• NCBI GeneID: 54756
• NCBI Accession #: NP_060033.3
• NCBI GenBank Nucleotide #: NM_017563.4
• UniProt Accession #: Q8NFM7; Q2NKP7; Q58EZ7; Q6RVF4; Q6UWI5; Q8N113; Q8NFS0; Q9UFA0
• NCBI Official Full Name: interleukin-17 receptor D isoform a
• NCBI Official Synonym Full Names: interleukin 17 receptor D
• NCBI Official Symbol: IL17RD
• NCBI Official Synonym Symbols: SEF; HH18; IL-17RD; IL17RLM
• NCBI Protein Information: interleukin-17 receptor D
• UniProt Protein Name: Interleukin-17 receptor D
• Protein Family: Interleukin-17 receptor
• UniProt Gene Name: IL17RD
• UniProt Synonym Gene Names: IL17RLM; SEF; IL-17 receptor D; IL-17RD; hSef

NCBI Description

This gene encodes a membrane protein belonging to the interleukin-17 receptor (IL-17R) protein family. The encoded protein is a component of the interleukin-17 receptor signaling complex, and the interaction between this protein and IL-17R does not require the interleukin. The gene product also affects fibroblast growth factor signaling, inhibiting or stimulating growth through MAPK/ERK signaling. Alternate splicing generates multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2016]

Uniprot Description

IL17RD: Feedback inhibitor of fibroblast growth factor mediated Ras-MAPK signaling and ERK activation. May inhibit FGF-induced FGFR1 tyrosine phosphorylation. Regulates the nuclear ERK signaling pathway by spatially blocking nuclear translocation of activated ERK without inhibiting cytoplasmic phosphorylation of ERK. Mediates JNK activation and may be involved in apoptosis. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: Golgi apparatus; Golgi membrane; integral to plasma membrane; nucleoplasm

Molecular Function: interleukin-17 receptor activity

Biological Process: MAPKKK cascade

Disease: Hypogonadotropic Hypogonadism 18 With Or Without Anosmia