SDS-PAGE
Dkk-3 / DKK3 recombinant protein
Recombinant Mouse Dkk-3 / DKK3 Protein (His tag)
Gene Names
Dkk3; C87148; AW061014
Purity
> 97 % as determined by SDS-PAGE
Synonyms
Dkk-3 / DKK3; Recombinant Mouse Dkk-3 / DKK3 Protein (His tag); AW061014; C87148; Dkk3; Dkk-3 / DKK3 recombinant protein
Host
Human Cells
Purity/Purification
> 97 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
349
Application Notes
The secreted recombinant mouse DKK3 consists of 338 amino acids and has a calculated molecular mass of 38 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 65 kDa protein in SDS-PAGE under reducing conditions.
Predicted N Terminal
Pro 23
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for Dkk-3 / DKK3 recombinant protein
Background: DKK3 (dickkopf related protein 3) is a member of the dickkopf-related family consisting of DKK1, DKK2, DKK3 and DKK4. It is a secreted protein, and also known as REIC (Reduced Expansion in Immortalized Cells). The DKK3 protein is proposed to function as a secreted tumor suppressor since it is downregulated in a number of cancer cells and prostate cancer tissue and may be a promising candidate molecule for therapeutic interference. DKK3 protein is also a negative regulator of beta-catenin and its downregulation contribute to an activation of the beta-catenin signaling pathway.
Description: A DNA sequence encoding the extracellular domain (Met 1-Ile 349) of mouse DKK3 (NP_056629.1) precursor was expressed with a C-terminal polyhistidine tag.
Description: A DNA sequence encoding the extracellular domain (Met 1-Ile 349) of mouse DKK3 (NP_056629.1) precursor was expressed with a C-terminal polyhistidine tag.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,388 Da
NCBI Official Full Name
dickkopf-related protein 3
NCBI Official Synonym Full Names
dickkopf WNT signaling pathway inhibitor 3
NCBI Official Symbol
Dkk3
NCBI Official Synonym Symbols
C87148; AW061014
NCBI Protein Information
dickkopf-related protein 3
UniProt Protein Name
Dickkopf-related protein 3
UniProt Gene Name
Dkk3
UniProt Synonym Gene Names
Dickkopf-3; Dkk-3; mDkk-3
Uniprot Description
DKK3: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Belongs to the dickkopf family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 7|7 F1
Cellular Component: extracellular region; extracellular space
Biological Process: negative regulation of aldosterone biosynthetic process; negative regulation of transcription, DNA-dependent
Research Articles on Dkk-3 / DKK3
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Product Notes
The Dkk-3 / DKK3 dkk3 (Catalog #AAA2547079) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The secreted recombinant mouse DKK3 consists of 338 amino acids and has a calculated molecular mass of 38 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 65 kDa protein in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the Dkk-3 / DKK3 dkk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dkk-3 / DKK3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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