CXCL11 elisa pair set

CXCL11 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118315

• Synonyms: CXCL11; CXCL11 Matched ELISA Antibody Pair Set; Human; Human I-TAC/CXCL11 ELISA Pair Set; chemokine (C-X-C motif) ligand 11; b-R1 Matched ELISA Antibody Pair Set; H174 Matched ELISA Antibody Pair Set; I-TAC Matched ELISA Antibody Pair Set; IP-9 Matched ELISA Antibody Pair Set; IP9 Matched ELISA Antibody Pair Set; SCYB11 Matched ELISA Antibody Pair Set; SCYB9B Matched ELISA Antibody Pair Set; CXCL11 elisa pair set

• Specificity: Quantitative determination of Human CXCL11

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 12.5-800pg/mL
• Sensitivity: 12.5pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of mouse anti-Human I-TAC/CXCL11 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human I-TAC/CXCL11 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 35 ng of recombinant Human I-TAC/CXCL11. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 800 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for CXCL11 elisa pair set

I-TAC, also known as CXCL11, is a small cytokine belonging to the CXC chemokine family. It is highly expressed in peripheral blood leukocytes, pancreas and liver, with moderate levels in thymus, spleen and lung and low expression levels were in small intestine, placenta and prostate. The I-TAC chemokine elicits its effects on its target cells by interacting with the cell surface chemokine receptor CXCR3, with a higher affinity than do the other ligands for this receptor, CXCL9 and CXCL10. I-TAC is chemotactic for activated T cells. The CXCL11 gene is located on human chromosome 4 along with many other members of the CXC chemokine family.

This CXCL11 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human CXCL11. It contains Human CXCL11 capture antibody, Human CXCL11 detector antibody (HRP) and a highly purified E Coli-expressed recombinant Human CXCL11 protein.

References

Strausberg RL, et al. (2003) Generation and initial analysis of more than 15, 000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903. Rani MR, et al. (2002) Requirement of phosphoinositide 3-kinase and Akt for interferon-beta-mediated induction of the beta-R1 (SCYB11) gene. J Biol Chem. 277(41): 38456-61. Salmaggi A, et al. (2003) Expression and modulation of IFN-gamma-inducible chemokines (IP-10, Mig, and I-TAC) in human brain endothelium and astrocytes: possible relevance for the immune invasion of the central nervous system and the pathogenesis of multiple sclerosis. J Interferon Cytokine Res. 22(6):631-40.