Testing Data
CD5L elisa pair set
CD5L Matched ELISA Antibody Pair Set, Mouse
Synonyms
CD5L; CD5L Matched ELISA Antibody Pair Set; Mouse; Mouse CD5L ELISA Pair Set; CD5 molecule-like; 42010 Matched ELISA Antibody Pair Set; AAC-11 Matched ELISA Antibody Pair Set; AI047839 Matched ELISA Antibody Pair Set; Api6 Matched ELISA Antibody Pair Set; CT2 Matched ELISA Antibody Pair Set; Pdp Matched ELISA Antibody Pair Set; Sp-alpha Matched ELISA Antibody Pair Set; CD5L elisa pair set
Specificity
Quantitative determination of Mouse CD5L
Assay Type
Solid Phase Sandwich ELISA
Detection Range
6.25-400pg/mL
Sensitivity
6.25pg/ml
Materials Provided
1. Capture Antibody 0.5 mg/mL of rabbit anti-mouse CD5L monoclonal antibody, Dilute to a working concentration of 2.0ug/mL in CBS before coating.
2. Detection Antibody 0.25 mg/mL rabbit anti-mouse CD5L polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 30 ng of recombinant mouse CD5L. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.
2. Detection Antibody 0.25 mg/mL rabbit anti-mouse CD5L polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 30 ng of recombinant mouse CD5L. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for CD5L elisa pair set
CD5L, also known as CD5 antigen-like, is a soluble protein belonging to group B of the scavenger receptor cysteine-rich (SRCR) superfamily and contains three SRCR domains. It is a secreted glycoprotein and expressed by macrophages presentin lymphoid tissues (spleen, lymph node, thymus, and bone marrow). It binds to myelomonocytic and lymphoid cells and may play an important role in the regulation of the innate and adaptive immune systems. CD5L functions as a pattern recognition molecule by binding both lipoteichoic acid (LTA) on Gram positive and lipopolysaccharide (LPS) on Gram negative bacteria. and the SRCR domain 1 of CD5L retains both the LPS and LTA binding activities. In addtion, it is revealed that CD5L seems to play a role as an inhibitor of apoptosis.
This CD5L Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse CD5L. It contains Mouse CD5L capture antibody, Mouse CD5L detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse CD5L protein.
This CD5L Matched ELISA Antibody Pair Set, Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse CD5L. It contains Mouse CD5L capture antibody, Mouse CD5L detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse CD5L protein.
References
Resnick, D. et al., 1994, Trends Biochem. Sci. 19: 5-8. Gebe, J. A. et al., 1997, J. Biol. Chem. 272 (10): 6151-6158. Sarrias, M.R. et al., 2004, Crit. Rev. Immunol. 24: 1-37. Mukhopadhyay, S. and Gordon, S., 2004, Immunobiology 209: 39-49. Sarrias, M. R. et al., 2005, J. Biol. Chem. 280 (42): 35391-35398.
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Product Notes
The CD5L (Catalog #AAA8118463) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CD5L, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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