Western Blot (WB)
(Western blot analysis of NCOA7 Antibody (N-term) in 293(lane 1), MDA-MB435(lane 2) cell line lysates (35ug/lane). NCOA7 (arrow) was detected using the purified Pab.)
Rabbit anti-Human NCOA7 Polyclonal Antibody | anti-NCOA7 antibody
NCOA7, NT (NCOA7, ERAP140, ESNA1, Nuclear receptor coactivator 7, 140kD estrogen receptor-associated protein, Estrogen nuclear receptor coactivator 1) (APC)
Gene Names
NCOA7; ESNA1; TLDC4; ERAP140; Nbla00052; Nbla10993; dJ187J11.3
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
NCOA7; Polyclonal Antibody; NT (NCOA7; ERAP140; ESNA1; Nuclear receptor coactivator 7; 140kD estrogen receptor-associated protein; Estrogen nuclear receptor coactivator 1) (APC); Estrogen nuclear receptor coactivator 1; anti-NCOA7 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-NCOA7 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
NCOA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82~112 amino acids from the N-terminal region of human NCOA7.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of NCOA7 Antibody (N-term) in 293(lane 1), MDA-MB435(lane 2) cell line lysates (35ug/lane). NCOA7 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of NCOA7 Antibody (N-term) in 293(lane 1), MDA-MB435(lane 2) cell line lysates (35ug/lane). NCOA7 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with NCOA7 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with NCOA7 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(NCOA7 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(NCOA7 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-NCOA7 antibody
NCOA7 enhances the transcriptional activities of several nuclear receptors. It is involved in the coactivation of different nuclear receptors, such as ESR1, THRB, PPARG and RARA.
Product Categories/Family for anti-NCOA7 antibody
References
Arai, H., et al. Oncol. Rep. 19(6):1381-1388(2008). Liu, Y., et al. Schizophr. Res. 89 (1-3), 360-361 (2007). Durand, M., et al. BMC Cell Biol. 8, 13 (2007). Mungall, A.J., et al. Nature 425(6960):805-811(2003). Ohira, M., et al. Cancer Lett. 197 (1-2), 63-68 (2003). Rushing, S.R., et al. Arch. Biochem. Biophys. 403(2):189-201(2002)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
106,162 Da
NCBI Official Full Name
nuclear receptor coactivator 7 isoform 2
NCBI Official Synonym Full Names
nuclear receptor coactivator 7
NCBI Official Symbol
NCOA7
NCBI Official Synonym Symbols
ESNA1; TLDC4; ERAP140; Nbla00052; Nbla10993; dJ187J11.3
NCBI Protein Information
nuclear receptor coactivator 7; putative protein product of Nbla00052; putative protein product of Nbla10993; TBC/LysM-associated domain containing 4; estrogen nuclear receptor coactivator 1; 140 kDa estrogen receptor-associated protein; estrogen receptor
UniProt Protein Name
Nuclear receptor coactivator 7
Protein Family
UniProt Gene Name
NCOA7
UniProt Synonym Gene Names
ERAP140; ESNA1
UniProt Entry Name
NCOA7_HUMAN
Uniprot Description
NCOA7: Enhances the transcriptional activities of several nuclear receptors. Involved in the coactivation of different nuclear receptors, such as ESR1, THRB, PPARG and RARA. Belongs to the OXR1 family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 6q22.32
Cellular Component: intracellular; nucleus
Molecular Function: protein binding; ligand-dependent nuclear receptor transcription coactivator activity; nuclear hormone receptor binding
Biological Process: transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter
Research Articles on NCOA7
Similar Products
Product Notes
The NCOA7 ncoa7 (Catalog #AAA6324211) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCOA7, NT (NCOA7, ERAP140, ESNA1, Nuclear receptor coactivator 7, 140kD estrogen receptor-associated protein, Estrogen nuclear receptor coactivator 1) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NCOA7 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NCOA7 ncoa7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCOA7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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